Myocardial tissue troponins T and I. An immunohistochemical study in experimental models of myocardial ischemia.

Background: Cardiac troponins T (cTnT) and I (cTnI) are proven diagnostic and risk stratification biomarkers in patients with acute coronary syndromes. To date, no immunohistochemical studies have been performed which allow visualization of the time course and pattern of myocardial troponin egress from the myocardium during the early evolution of ischemic injury in experimental systems.

Methods: We studied archival formalin-fixed, paraffin-embedded myocardium from 50 experimental animals (dogs, pigs and rats) that had undergone permanent coronary occlusion (n = 34) for 0.