Publications by authors named "Maria Manzan"
Article Synopsis
- Most variations in how sensitive organisms are to TCDD toxicity, like suppressed antibody responses, stem from genetic differences (SNPs) within the aryl hydrocarbon receptor (AhR) gene, especially noted in murine models.
- The specific reasons for sensitivity differences in humans are not fully understood but involve different polymorphic forms of the AhR gene across individuals.
- In this study, it was found that the R554K SNP reduced gene expression and activity in human B cells in response to TCDD, while a combination of three SNPs (P517S, R554K, V570I) was needed to lessen the suppression of IgM antibody secretion by TCDD.
B cell differentiation and humoral immune responses are markedly suppressed by the persistent environmental contaminant, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The suppression of humoral immune responses by TCDD occurs by direct actions on the B cell and involves activation of the aryl hydrocarbon receptor. Transcriptional regulation of paired box gene 5 (Pax5), an important regulator of B cell differentiation, is altered by TCDD in concordance with the suppression of B cell differentiation and humoral immunoglobulin M response.
View Article and Find Full Text PDFA specific multiplex PCR was developed for the rapid and highly sensitive quality control of the viral DNA during Epinotia aporema granulovirus (EpapGV) production. At the beginning of this work only 2.3% of the EpapGV genomic sequence was known.
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- A new cell line called MSU-TnT4 (TnT4) was created from Trichoplusia ni embryos to test its capabilities in producing membrane proteins using baculovirus expression vectors.
- The TnT4 cells showed a longer doubling time of 21 hours but produced the human neurotensin receptor 1-GFP fusion protein at about twice the level compared to Sf21 cells.
- Overall, using this new cell line with recombinant baculoviruses offers a quick way to assess the effectiveness of various cell lines in producing membrane proteins through the baculovirus expression vector system (BEVS).
The persistent environmental contaminant and immunotoxicant, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), markedly suppresses humoral immune responses. We recently reported impaired down-regulation of paired box 5 (Pax5), a repressor of B cell differentiation and concomitant suppression of the IgM response by TCDD in the murine CH12.LX B cell line.
View Article and Find Full Text PDFThe ecdysteroid UDP-glycosyltransferase (egt) gene of Epinotia aporema granulovirus (EpapGV) was cloned sequenced and its biological activity was assessed. It encodes a protein of 446 amino acids. Direct evidence that the cloned gene encodes an active EGT protein was obtained by transient expression assays in insect cells.
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