Publications by authors named "Maria Luiza Barucci Araujo Pires"
Objective: To investigate the effects of quercetin (QU), hesperetin (HT), and taxifolin (TX) on human dental pulp cells (hDPCs) chronically exposed to lipopolysaccharide (LPS).
Methods: First, the cytotoxicity (alamarBlue) and bioactivity (biomineralization, Alizarin Red) of QU, HT, and TX concentrations were evaluated on healthy hDPCs. Then, the effects of non-cytotoxic and bioactive concentrations were investigated on hDPCs after previous stimulation with E.
Objective: This study investigated the bioactive effects of retinoic acid and ascorbic acid on hSCAPs in vitro.
Design: Cells were obtained from human third molars (n=4) and characterized for mesenchymal stem cell markers by flow cytometry. The experimental groups: control (α-MEM); vehicle control group (α-MEM + 0.
Objectives: To investigate the transdentinal effects of surface reaction-type pre-reacted glass-ionomer (S-PRG) fillers on odontoblast-like cells.
Methods: An eluate of S-PRG fillers was obtained by dissolving the particles in distilled water (1:1 m/v). Dentin discs with similar permeability were mounted into artificial pulp chambers and MDPC-23 cells were seeded on their pulpal surface.
Influence of coating dental enamel with a TiF-loaded polymeric primer on the adverse effects caused by a bleaching gel with 35% HO.
J Mech Behav Biomed Mater
May 2024
Article Synopsis
- The study aimed to determine if using a polymeric primer containing titanium tetrafluoride improves the effectiveness of a bleaching gel on tooth enamel, while also protecting enamel's structure and reducing toxicity.
- Standardized enamel/dentin discs were treated with varying concentrations of titanium tetrafluoride before applying a 35% bleaching gel, and several tests were conducted to measure bleaching efficacy, enamel hardness, and cytotoxic effects.
- Results showed that while the primer significantly improved bleaching outcomes (with group 10TiF showing the best results), it also helped to limit damage to the enamel's microstructure compared to the untreated control.
Objectives: To investigate the response of pulp cells to the application of silver diamine fluoride (SDF) and potassium iodide (KI) on demineralized dentin.
Materials And Methods: The occlusal surfaces of human dentin discs (0.4 mm thick) with similar permeability were subjected to an artificial caries protocol, and then the discs were adapted into artificial pulp chambers.
Chronic exposure to lipopolysaccharides as an in vitro model to simulate the impaired odontogenic potential of dental pulp cells under pulpitis conditions.
J Appl Oral Sci
July 2023
Article Synopsis
- Researchers aimed to understand how chronic exposure to lipopolysaccharides (LPS) affects human dental pulp cells (HDPCs) and their ability to regenerate tissue under pulpitis conditions.
- They conducted experiments by exposing HDPCs to various concentrations of LPS, analyzing cellular responses such as gene expression, inflammation markers, and cell viability over time.
- Results showed that higher LPS concentrations led to inflammation, reduced gene expression related to tooth development, and decreased cell viability and mineralization, indicating a negative impact on the cells' regenerative potential.
Objectives: To evaluate the inhibitory activity of an ion-releasing filler (S-PRG) eluate on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation.
Methods: Dentin beams (5 × 2 × 0.5 mm) from human molars were completely demineralized to produce dentin matrix specimens.