Publications by authors named "Maria Luisa Nunes Diehl"

Acanthamoeba spp. are among the most worldwide prevalent protozoa. It is the causative agent of a disease known as Acanthamoeba keratitis, a painful and severe sight-threatening corneal infection that can lead to blindness.

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Article Synopsis
  • An alternative method for cloning amoebas is introduced, which involves scraping cells from agar.
  • The cells are then placed in a new agar medium divided into isolated squares to prevent them from mixing.
  • This technique improves the reliability of cloning and allows for better quality control during the process.
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Background: Leishmaniasis reaches millions of people around the world. The control of the disease is difficult due to the restricted access to the diagnosis and medication, and low adherence to the treatment. Thus, more efficient drugs are needed and natural products are good alternatives.

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