Implantation of heat treatment Ti6al4v alloys in femoral bone of Wistar rats.

Two heat treatments were carried out at below (Ti6Al4V) and above (Ti6Al4V) the beta-phase transformation temperature (T = 980 °C), to study the effect of microstructural changes on osseointegration. The alloys were implanted in the femurs of hind legs of Wistar rats for 15, 30, and 60 days. Histology of the femur sections obtained for the first 15 days showed inflammatory tissue surrounding the implants and tissue contraction, which prevented osseointegration in early stages.

Effect of the Heat-Treated Ti6Al4V Alloy on the Fibroblastic Cell Response.

Two heat treatments were carried out below (Ti6Al4V) and above (Ti6Al4V) Ti6Al4V beta-phase transformation temperature (980 °C), with the purpose of studying the effect of microstructure on the adhesion and proliferation of fibroblast cells, as well as their electrochemical behavior. These alloys were seeded with 10,000 L929 fibroblast cells and immersed for 7 days in the cell culture at 37 °C, pH 7.40, 5% CO₂ and 100% relative humidity.

Osteoblast Cell Response on the Ti6Al4V Alloy Heat-Treated.

In an effort to examine the effect of the microstructural changes of the Ti6Al4V alloy, two heat treatments were carried out below (Ti6Al4V) and above (Ti6Al4V) its β-phase transformation temperature. After each treatment, globular and lamellar microstructures were obtained. Saos-2 pre-osteoblast human osteosarcoma cells were seeded onto Ti6Al4V alloy disks and immersed in cell culture for 7 days.