Publications by authors named "Maria Lopez Martinez"

Article Synopsis
  • Recent research on late-life dementia highlights both neurodegenerative and vascular conditions contributing to the varied symptoms in patients, utilizing the Vallecas Alzheimer's Reina Sofía (VARS) cohort with over 550 participants and associated brain donations.
  • The study focused on 167 patients, primarily older women (79%) with an average age of 88, and found a significant presence of the ApoE-e4 gene variant (43%).
  • The main findings revealed that 79.6% had Alzheimer's disease, with a high prevalence of other conditions like vascular dementia and Lewy body dementia, and over 71.1% of the patients showed multiple neuropathological findings, indicating the complexity of dementia in this cohort.
View Article and Find Full Text PDF

(1) Reduced glutathione (GSH) is considered the first line of antioxidant defense. During oxidative stress, it is oxidized to glutathione disulphide (GSSG). (2) A simple and quick spectrophotometric method based on sodium borohydride (NaBH) as a reductant to measure the total and reduced GSH in porcine saliva was analytically validated and evaluated in two situations in this species: (a) in a physiological situation, involving sows during the late lactation and post-weaning periods, and (b) in a situation of sepsis in pigs experimentally induced by LPS administration.

View Article and Find Full Text PDF

Cystatin C, ammonia, and bicarbonate have been described to be biomarkers of sepsis and inflammation in humans. The saliva of pigs can be used to detect a wide range of pathogens but also many biomarkers that can be analyzed to evaluate different conditions such as stress (i.e.

View Article and Find Full Text PDF

T cells protect tissues from cancer. Although investigations in mice showed that amino acids (AA) critically regulate T cell immunity, this remains poorly understood in humans. Here, we describe the AA composition of interstitial fluids in keratinocyte-derived skin cancers (KDSCs) and study the effect of AA on T cells using models of primary human cells and tissues.

View Article and Find Full Text PDF
Article Synopsis
  • - The study examined how different handling conditions at a slaughterhouse affect salivary biomarkers in finishing pigs, comparing a low-stress group (Group A) to a high-stress group (Group B).
  • - Saliva samples were collected before and after transport, with Group B showing significantly higher levels of stress-related biomarkers, such as cortisol and haptoglobin, compared to Group A.
  • - The findings suggest that managing stress during handling can alter salivary biomarkers, indicating the potential for using these biomarkers to monitor animal welfare at slaughter.
View Article and Find Full Text PDF

The objective of the current study was to adapt and validate the pure procrastination scale (PPS) for the Spanish adult population. Procrastination can have numerous consequences in daily life, making it essential to have reliable and valid instruments for measuring procrastination. Thus, this study was conducted to address this need.

View Article and Find Full Text PDF

Equine gastric ulcer syndrome (EGUS) is currently one of the more frequent diseases in horses. We aimed to identify changes in the salivary proteome in horses with EGUS at diagnosis and after successful treatment by using gel proteomics. Saliva samples were collected from nine horses with EGUS before and after treatment and nine matched healthy controls.

View Article and Find Full Text PDF

The use of saliva as a biological sample from pigs is of high practical interest because blood collection from pigs is difficult and stressful. In this study, the influence of two different materials, a cotton roll and a polypropylene sponge, in porcine saliva collection was evaluated. For this purpose, the effect of the material used for sampling was evaluated in a panel of 13 analytes, including those related to stress (cortisol and oxytocin), inflammation and immunity (adenosine deaminase, haptoglobin and myeloperoxidase), redox homeostasis (the cupric reducing ability of saliva, the ferric reducing activity of saliva, and the Trolox equivalent antioxidant capacity), and sepsis (procalcitonin), as well as other routine analytes related to metabolism and different tissues and organs, such as lactate dehydrogenase, creatine kinase, urea, and total protein concentration.

View Article and Find Full Text PDF
Article Synopsis
  • * Traditional animal models are limited and push researchers to explore more relevant alternatives, leading to advancements in technology such as organoids and organ-on-a-chip models.
  • * The review highlights innovative methods like microfluidics, 3D cell cultures, and the use of induced pluripotent stem cells (iPSCs) to create better models that mimic healthy and diseased neural tissue.
View Article and Find Full Text PDF

In the last few decades, there has been increasing social awareness for environmental conservation, which is driving the development of composite materials based on natural fibers. These new materials have interesting properties that allow for their use in a variety of applications. This study deals with the development of composite materials based on unsaturated polyester resins reinforced with recycled mineral fibers, such as slate fibers obtained from slate production waste, which have similar properties to glass fiber.

View Article and Find Full Text PDF

An assay for the measurement of myeloperoxidase (Mpx) in porcine saliva was developed and validated, and factors influencing Mpx and another two biomarkers of inflammation and immune system, the protein S100A12 and the inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4), were studied. The spectrophotometric method for Mpx measurement validated in this assay showed an adequate analytical performance including precision and accuracy. When a group of twenty healthy pigs was sampled every 4 h from 8 a.

View Article and Find Full Text PDF
Article Synopsis
  • * Researchers sampled pigs from 18 farms at various ages, collecting a total of 504 OF samples, and analyzed them for 11 biomarkers including cortisol and haptoglobin.
  • * Findings showed that OF collected with ropes was dirtier and had higher levels of certain biomarkers compared to sponge-collected samples, indicating that sponges may be the better method for obtaining cleaner samples for analysis.
View Article and Find Full Text PDF
Article Synopsis
  • The study focuses on calgranulins (S100A8, S100A9, and S100A12), proteins linked to inflammation and immune responses, as possible biomarkers in pigs with diarrhea.
  • Researchers measured these proteins alongside other analytes related to inflammation, immunity, stress, tissue damage, and sepsis to understand their behavior in affected pigs.
  • Results showed that levels of S100A8/A9 and A12 increased in the saliva of pigs with diarrhea, correlating significantly with other analyzed substances, suggesting potential for further research on their use as biomarkers.
View Article and Find Full Text PDF

Background: Streptococcus suis (S. suis) is a Gram-positive bacteria that infects pigs causing meningitis, arthritis, pneumonia, or endocarditis. This increases the mortality in pig farms deriving in severe economic losses.

View Article and Find Full Text PDF

S100 proteins are a group of calcium-binding proteins which received this name because of their solubility in a 100% saturated solution of ammonium sulphate. They have a similar molecular mass of 10-12 KDa and share 25-65% similarity in their amino acid sequence. They are expressed in many tissues, and to date 25 different types of S100 proteins have been identified.

View Article and Find Full Text PDF

represents the main cause of diarrhoea in pigs. Saliva can provide information about the pathophysiology of diseases and be a source of biomarkers. We aimed to identify changes in the salivary proteome of pigs with diarrhoea caused by .

View Article and Find Full Text PDF
Article Synopsis
  • Calprotectin (CALP) is a protein complex involved in inflammation and can be used as a biomarker for inflammatory conditions like sepsis, measurable in various human fluids.
  • This study focused on validating an automated assay to measure CALP in pig saliva, offering a non-invasive and simple collection method.
  • The results revealed that CALP levels in pig saliva fluctuated throughout the day and increased significantly after exposure to lipopolysaccharide and stress, indicating potential use as a health and welfare indicator for pigs.
View Article and Find Full Text PDF

Three-dimensional (3D) bioprinting is a promising technique for the development of neuronal models because it controls the deposition of materials and cells. Finding a biomaterial that supports neural differentiation while ensuring compatibility with the technique of 3D bioprinting of a self-standing construct is a challenge. In this study, gelatin methacryloyl (GelMA), methacrylated alginate (AlgMA), and hyaluronic acid (HA) were examined by exploiting their biocompatibility and tunable mechanical properties to resemble the extracellular matrix (ECM) and to create a suitable material for printing neural progenitor cells (NPCs), supporting their long-term differentiation.

View Article and Find Full Text PDF

Meningitis due to causes high mortality and morbidity on pig farms and has increasing zoonotic potential worldwide. Saliva proteome analysis would potentially be useful in elucidating pathophysiological changes and mining for new biomarkers to diagnose and monitor infection. The objective of this study was to investigate the changes in the salivary and serum proteome profile of piglets with meningitis.

View Article and Find Full Text PDF

There is a need for feasible and reliable measures to improve and evaluate production animal health and welfare. Oxytocin is a promising novel stress-related biomarker and procalcitonin may be a measure of sepsis. Both have potential for use in pigs and can be measured from saliva, which allows on-farm sampling with minimal impact on the animals.

View Article and Find Full Text PDF
Article Synopsis
  • Saliva from pigs is being recognized as a useful sample for monitoring their health and welfare through various biomarkers.
  • This study focused on how salivary biomarkers related to redox status change in pigs that were intentionally made septic and compared them with healthy and non-septic inflamed pigs.
  • Results showed that certain biomarkers significantly increased in the saliva of septic pigs over time, indicating that these changes could potentially serve as indicators of sepsis in pigs.
View Article and Find Full Text PDF

Sepsis is a systemic inflammatory response triggered by an infectious agent and is recognized by the World Health Organization as a global concern, since it is one of the major causes of severe illness in humans and animals. The study of the changes that can occur in saliva and serum in sepsis can contribute to a better understanding of the pathophysiological mechanisms involved in the process and also to discover potential biomarkers that can help in its diagnosis and monitoring. The objective of this study was to characterize the changes that occur in the salivary and serum proteome of pigs with experimentally-induced sepsis.

View Article and Find Full Text PDF

Over the most recent decades, the development of new biological platforms to study disease progression and drug efficacy has been of great interest due to the high increase in the rate of neurodegenerative diseases (NDDs). Therefore, blood-brain barrier (BBB) as an organ-on-a-chip (OoC) platform to mimic brain-barrier performance could offer a deeper understanding of NDDs as well as a very valuable tool for drug permeability testing for new treatments. A very attractive improvement of BBB-oC technology is the integration of detection systems to provide continuous monitoring of biomarkers in real time and a fully automated analysis of drug permeably, rendering more efficient platforms for commercialization.

View Article and Find Full Text PDF

Background: Procalcitonin (PCT) is a widely used biomarker of sepsis in human medicine and can have potential applications in the veterinary field. This study aimed to explore whether PCT could be measured in the saliva of pigs and whether its concentration changes in sepsis. Therefore, a specific assay was developed and analytically validated, and changes in PCT concentration were evaluated in two conditions: a) in an experimental model of sepsis produced by the administration of lipopolysaccharide (LPS) to pigs (n = 5), that was compared with a model of non-septic inflammation induced by turpentine oil (n = 4), and b) in healthy piglets (n = 11) compared to piglets with meningitis (n = 20), a disease that usually involves sepsis and whose treatment often requires large amounts of antibiotics in farms.

View Article and Find Full Text PDF

Background: Clinical diagnosis of atypical parkinsonisms may be challenging. The eye-of-the-tiger sign on brain MRI, typical of neurodegeneration with brain iron accumulation, has been anecdotally observed in cases clinically diagnosed as atypical parkinsonisms.

Objectives: To show how clinical syndromes and even neuroimaging sometimes may lead the neurologist to a misunderstanding, just as to emphasize the important role of pathology to establish the final diagnosis in these cases.

View Article and Find Full Text PDF

A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_sessionr66grkht6iersl2r02e1bgpoivod1frg): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once