Publications by authors named "Maria Laura Olivares"
Article Synopsis
- This research focuses on creating albumin-based nanoparticles using microfluidic techniques for advanced nutritional products.
- By using simple equipment for solvent-shifting nanoprecipitation, the study successfully produced curcumin-loaded alpha lactalbumin nanoparticles.
- The final nanoparticles showed a uniform size, with a 40% efficiency of curcumin entrapment and a gradual release of nearly 70% of the bioactive compound in experiments.
In the present work, we have determined the effect of expression vectors and their corresponding host bacteria on the antigenic performance of Trypanosoma cruzi P2beta (TcP2beta) full-length recombinant protein. The gene encoding the TcP2beta ribosomal protein was cloned in pMAL-c2 and pET-32a vectors that allow the expression of high levels of soluble fusion proteins. A panel of 32 positive and 32 negative sera was assayed with the purified proteins expressed using pMal-c2 (TcP2beta-MBP) and pET-32a (TcP2beta-TRX) vectors and with MBP and TRX purified from pMAL-c2 and pET-32a vectors, respectively.
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