Conserved MicroRNAs in Human Nasopharynx Tissue Samples from Swabs Are Differentially Expressed in Response to SARS-CoV-2.

The use of high-throughput small RNA sequencing is well established as a technique to unveil the miRNAs in various tissues. The miRNA profiles are different between infected and non-infected tissues. We compare the SARS-CoV-2 positive and SARS-CoV-2 negative RNA samples extracted from human nasopharynx tissue samples to show different miRNA profiles.

A novel mitovirus detected in Diaporthe rudis, a fungus associated with Phomopsis dieback on grapevines.

The genome sequence of a mitovirus found in an isolate of Diaporthe rudis, one of the causal agents of Phomopsis dieback on grapevines, was determined by two high-throughput sequencing approaches, small RNA and total RNA sequencing. The genome of this mitovirus is 2,455 nt in length and includes a single large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp). A BLASTx comparison of the full-length genome sequence showed the highest similarity (54.