A continuous in silico learning strategy to identify safety liabilities in compounds used in the leather and textile industry.

There is a widely recognized need to reduce human activity's impact on the environment. Many industries of the leather and textile sector (LTI), being aware of producing a significant amount of residues (Keßler et al. 2021; Liu et al.

Evaluation of Asphalt Mixtures Containing Metallic Fibers from Recycled Tires to Promote Crack-Healing.

This paper reports part of an international research project with the long-term aim of developing more sustainable asphalt mixture with crack-healing properties by the addition of recycled metallic waste from industrial sources. Specifically, this article presents an evaluation of the physical, thermophysical, and mechanical properties of asphalt mixtures with metallic fiber obtained from recycled tires for crack-healing purposes. Detailed results on the crack-healing of asphalt mixtures will be reported in a second article.

A Complementary Scale of Biased Agonism for Agonists with Differing Maximal Responses.

Compelling data in the literature from the recent years leave no doubt about the pluridimensional nature of G protein-coupled receptor function and the fact that some ligands can couple with different efficacies to the multiple pathways that a receptor can signal through, a phenomenon most commonly known as functional selectivity or biased agonism. Nowadays, transduction coefficients (log(τ/K)), based on the Black and Leff operational model of agonism, are widely used to calculate bias. Nevertheless, combining both affinity and efficacy in a single parameter can result in compounds showing a defined calculated bias of one pathway over other though displaying varying experimental bias preferences.

Approaches to Characterize and Quantify Oligomerization of GPCRs.

Fluorescence resonance energy transfer (FRET) is an approach widely used to detect protein-protein interactions in live cells. This approach is based on the sensitization of an "acceptor" molecule by the energy transfer from a "donor" when there is an overlap between the emission spectrum of the "donor" and the excitation spectrum of the "acceptor" and close proximity between the two fluorophore species (in the region of 8 nm). Various methods exist to quantify FRET signals: here, we describe the application of homogeneous time-resolved FRET (htrFRET) combined with Tag-lite™ technology and its application to determine not only protein-protein interactions but also the capability of GPCR mutant variants to form homomers compared to the wild type GPCR within the plasma membrane of transfected cells.

Prevalence of sleep disorders in children of a public hospital in São Paulo.

Objective: To investigate the prevalence of sleep disorders in a sample of children from a public hospital in the city of São Paulo, Brazil.

Method: 330 children, who came to the clinical laboratory, were consecutively investigated for sleep disorders, using the sleep disturbance scale for children. Gender, age, and social/economical classification were considered.