Publications by authors named "Maria Jose Hijarrubia"
Article Synopsis
- The alpha-aminoadipate reductase (alpha-AAR) of Penicillium chrysogenum plays a crucial role in activating alpha-aminoadipic acid and reducing it to alpha-aminoadipic semialdehyde through a series of enzymatic processes.
- The enzyme was purified using immunoaffinity techniques, and its kinetic properties with alpha-aminoadipic acid, ATP, and NADPH were analyzed, confirming the structure through N-terminal sequencing.
- Research into the enzyme’s domain structure revealed that proteolytic cleavage generates fragments linked to distinct functional domains, indicating that the peptidyl carrier protein (PCP) domain contributes to the stability of the acyl intermediate formed during the enzymatic reaction
