Effect of Caseinophosphopeptides from αs- and β-Casein on Iron Bioavailability in HuH7 Cells.

Two pools of caseinophosphopeptides (CPPs) obtained from αs- and β-casein fractions (α-CPPs and β-CPPs) were characterized. A total of 16 CPPs were identified in the α-CPPs pool, 9 of them derived from αs1-casein and 7 from αs2-casein. A total of 18 CPPs were identified in the β-CPPs pool.

Antioxidant activity and protective effects of peptide lunasin against oxidative stress in intestinal Caco-2 cells.

Oxidative stress is one of the most critical factors implicated in many disorders of the gastrointestinal tract, such as inflammatory bowel diseases and colon cancer. Lunasin is a seed peptide known by its properties against cancer and cardiovascular diseases. The present study investigated the in vitro antioxidant activity of this peptide that demonstrated to be a potent scavenger of peroxyl and superoxide radicals.

Iron and zinc bioavailability in Caco-2 cells: influence of caseinophosphopeptides.

A study has been made of the influence of two pools of caseinophosphopeptides (CPPs) obtained from α(s)- and β-casein (CN) fractions, and of three specific CPPs (β-CN(1-25)4P, α(s1)-CN(64-74)4P and α(s2)-CN(1-19)4P), on iron bioavailability (ferritin synthesis) and zinc bioavailability (retention, transport and uptake of zinc) in Caco-2 cells. α-CPP and β-CPP pools did not improve ferritin synthesis, but the three specific CPPs showed an increase in ferritin synthesis in Caco-2 cells versus iron sulphate, β-CN(1-25)4P being the most effective. In relation to zinc bioavailability, α-CPPs, β-CPPs, α(s1)-CN(64-74)4P and β-CN(1-25)4P increased zinc uptake.

Milk versus caseinophosphopeptides added to fruit beverage: resistance and release from simulated gastrointestinal digestion.

The influence of simulated gastrointestinal digestion on caseinophosphopeptides (CPPs) formation in milk-based fruit beverage was evaluated, together with resistance of a pool of CPPs added to fruit beverage. In milk-based fruit beverage, four CPPs were identified that can be justified by their presence in raw milk or due to processing. When it was subjected to simulated gastrointestinal digestion, 10 CPPs were identified, and only 1 presented the cluster (SpSpSpEE) (3 phosphoseryl group followed by 2 glutamic acid residues), which corresponded to alpha(s2)-CN(1-19)4P.