Effects of conceptus proteins on endometrium and blood leukocytes of dairy cattle using transcriptome and meta-analysis.

This study investigates the short and long-term effects of IFNT and PAG on the transcriptome of endometrium and blood leukocytes. Holstein heifers received intrauterine infusions of one of the following treatments: 20 mL of a 200 μg/mL bovine serum albumin solution (BSA; vehicle) from day 14 to 16 of the estrous cycle (BSA), vehicle + 10 μg/mL of IFNT from day 14 to 16 (IFNT3), vehicle + 10 μg/mL of IFNT from day 14 to 19 (IFNT6), and vehicle + 10 μg/mL of IFNT from day 14 to 16 followed by vehicle + 10 μg/mL of IFNT + 5 μg/mL of PAG from day 17 to 19 (IFNT+PAG). RNA-seq analysis was performed in endometrial biopsies and blood leukocytes collected after treatments.

Importance of body condition score and ovarian activity on determining the fertility in beef cows supplemented with long-acting progesterone after timed-AI.

The working hypothesis was that supplementation with progesterone (iP4) at early diestrus increases pregnancy rates of suckled beef cows with poor body condition score (BCS) and/or in anestrus when cows are submitted to timed insemination (TAI). The time of ovulation among suckled multiparous (n = 1270) and primiparous (n = 303) Nelore cows was synchronized using an estradiol/P4-based protocol for TAI (D0). At Day ‒10, visual evaluation of BCS was performed (scale = 1-5) and animals were classified according to an ovarian activity (OA) score: (OA1) absence of CL and presence of follicles ≥ 8 mm, (OA2) absence of CL and presence of follicles < 8 mm and (OA3) presence of corpus luteum (CL).

Morphology and Morphometry of the Ovaries and Uteri of the Amazonian Freshwater Stingrays (Potamotrygonidae: Elasmobranchii).

The reproductive biology of South American freshwater stingrays (family Potamotrygonidae) is still poorly studied compared to other marine species. In the present study, we examined the gross anatomy and histology of six species of potamotrygonids from the Amazon basin and described the structural asymmetry of the ovaries and their relationship between ovarian and uterine fecundities. Stereological techniques were used to quantify the volume of ovarian and epigonal organ tissue associated with the left and right sides of the Potamotrygon wallacei, a recently described species, locally known as the cururu ray.

Coronin-1 expression in T lymphocytes: insights into protein function during T cell development and activation.

Coronin has been described as an actin-binding protein of Dictyostelium discoideum, and it has been demonstrated to play a role in cell migration, cytokinesis and phagocytosis. Coronin-related proteins are found in many eukaryotic species, including Coronin-1 in mammals whose expression is enriched in the hematopoietic tissues. Here, we characterize Coronin-1 gene and protein expression in mouse embryonic and adult T lymphocytes.

Wdr12, a mouse gene encoding a novel WD-Repeat Protein with a notchless-like amino-terminal domain.

The WD-repeat protein family consists of a large group of structurally related yet functionally diverse proteins found predominantly in eukaryotic cells. These factors contain several (4-16) copies of a recognizable amino-acid sequence motif (the WD unit) thought to be organized into a "propeller-like" structure involved in protein-protein regulatory interactions. Here, we report the cloning of a mouse cDNA, referred to as Wdr12, which encodes a novel WD-repeat protein of 423 amino acids.