Temperature stability of urinary F-isoprostane and 8-hydroxy-2-deoxyguanosine.

Background: Clinical and epidemiological studies employ long-term temperature storage but the effect of temperature on the stability of oxidative stress (OS) markers is unknown. We investigated the effects of storage at -20 °C and -80 °C over 4-9 months on F-isoprostanes (F-IsoP) and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels in urine of children, a population group among whom the measurement of these markers is still limited.

Methods: Paired spot urine samples from 87 children aged 8.