Publications by authors named "Maria I Chavez-Bejar"
Background: Single-cell oils obtained from oleaginous microorganisms by using lignocellulosic waste hydrolysates are an alternative for producing biodiesel.
Aims: To isolate a yeast strain able to produce lipids from centrifuged nejayote (CN), hydrolyzed nejayote solids (HNS) and hydrolyzed sugarcane bagasse (HSB).
Methods: In order to identify the yeasts recovered, 26S ribosomal DNA was sequenced.
Article Synopsis
- Natural aromatic polymers like melanins have useful applications in cosmetics, pharmaceuticals, and chemicals, with production relying on converting L-tyrosine into melanins using tyrosinase.
- Researchers genetically engineered E. coli to enhance melanin production by expressing a mutated tyrosinase and modifying key metabolic pathways, ultimately allowing them to handle carbon sources effectively without inhibiting growth.
- The study successfully produced 3.22 g/L of melanin using glucose over 120 hours, showing that this method can be more cost-effective than traditional production methods that rely on L-tyrosine.
The expression of the feedback inhibition-insensitive enzyme cyclohexadienyl dehydrogenase (TyrC) from Zymomonas mobilis and the chorismate mutase domain from native chorismate mutase-prephenate dehydratase (PheA(CM)) from Escherichia coli was compared to the expression of native feedback inhibition-sensitive chorismate mutase-prephenate dehydrogenase (CM-TyrA(p)) with regard to the capacity to produce l-tyrosine in E. coli strains modified to increase the carbon flow to chorismate. Shake flask experiments showed that TyrC increased the yield of l-tyrosine from glucose (Y(l-Tyr/Glc)) by 6.
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