Phylogenetic analysis of vancomycin-resistant Enterococcus faecium genotypes associated with outbreaks or sporadic infections in Italy.

Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to investigate the genetic relatedness of a total of 41 Enterococcus faecium isolates from different backgrounds (hospital outbreaks, n = 9; documented sporadic infections, n = 10; asymptomatic sporadic carriage of hospitalized patients, n = 9; healthy persons, n = 3; non-human sources, n = 10) over the period 1996-2004 in comparison with clones that have spread in Italy since 1993. Thirty six isolates were vancomycin-resistant and five were vancomycin-susceptible. eBURST analysis of MLST sequence types generated two groups: (1) group 1 (27 isolates) forming a clonal complex (CC17) with the predicted founder corresponding to ST17, a genotype identified in 1994, that included esp-positive and -negative clones isolated from hospitalized patients; and (2) group 2 (14 isolates) including esp-negative clones from different sources (hospitalized patients, healthy persons and non-human sources).

Emergence of linezolid resistance in the vancomycin-resistant Enterococcus faecium multilocus sequence typing C1 epidemic lineage.

A relatively high rate of vancomycin-resistant Enterococcus faecium not susceptible to linezolid was observed in intensive care unit patients. Linezolid-resistant isolates carried the G2576T mutation in the 23S rRNA gene, belonged to different clones, and shared the same allelic profile, which clusters in the C1 multilocus sequence typing epidemic lineage.

Vancomycin-resistant Enterococcus faecium isolates causing hospital outbreaks in northern Italy belong to the multilocus sequence typing C1 lineage.

Multilocus sequence typing (MLST) was used to obtain insights into the genetic relationships between 14 vancomycin-resistant Enterococcus faecium (VREF) isolates from humans (hospitalized patients, 5 strains) and nonhuman sources (meat and poultry, 9 strains) in northern Italy over the period 1993-2001. The typing scheme (Homan et al., 2002, J.

The vanG glycopeptide resistance operon from Enterococcus faecalis revisited.

Acquired VanG-type resistance to vancomycin (MIC = 16 micro g ml(-1)) but susceptibility to teicoplanin in Enterococcus faecalis BM4518 and WCH9 is due to the inducible synthesis of peptidoglycan precursors ending in d-alanine-d-serine. The vanG cluster, assigned to a chromosomal location, was composed of genes recruited from various van operons. The 3' end encoded VanG, a d-Ala:d-Ser ligase, VanXY(G), a putative bifunctional d,d-peptidase and VanT(G), a serine racemase: VanG and VanT(G) were implicated in the synthesis of d-Ala:d-Ser as in VanC- and VanE-type strains.

Evaluation of the VITEK 2 system for identification and antimicrobial susceptibility testing of medically relevant gram-positive cocci.

A study was conducted to evaluate the new VITEK 2 system (bioMérieux) for identification and antibiotic susceptibility testing of gram-positive cocci. Clinical isolates of Staphylococcus aureus (n = 100), coagulase-negative staphylococci (CNS) (n = 100), Enterococcus spp. (n = 89), Streptococcus agalactiae (n = 29), and Streptococcus pneumoniae (n = 66) were examined with the ID-GPC identification card and with the AST-P515 (for staphylococci), AST-P516 (for enterococci and S.