(B), (C), and a New -Like Gene, (E), in Clostridium difficile Strains Recovered across Latin America.

Cfr is a radical -adenosyl-l-methionine (SAM) enzyme that confers cross-resistance to antibiotics targeting the 23S rRNA through hypermethylation of nucleotide A2503. Three -like genes implicated in antibiotic resistance have been described, two of which, (B) and (C), have been sporadically detected in However, the methylase activity of Cfr(C) has not been confirmed. We found (B), (C), and a -like gene that shows only 51 to 58% protein sequence identity to Cfr and Cfr-like enzymes in clinical isolates recovered across nearly a decade in Mexico, Honduras, Costa Rica, and Chile.