Hyperbaric oxygen therapy and ischemia and reperfusion: a valuable association to attenuate ischemic lesion and hepatic reperfusion.

Purpose: To investigate the consequences of the association between hyperbaric oxygen therapy and hepatic ischemia / reperfusion.

Methods: Wistar rats were divided into three groups: SHAM, rats submitted to surgical stress and anesthetic but not hepatic ischemia or reperfusion, I / R, rats submitted to total hepatic pedicle ischemia for 30 min, followed by 5 min of reperfusion; HBO120, rats submitted to 120 min of hyperbaric oxygen therapy at two absolute atmospheres and immediately after submitted to the experimental protocol of ischemia and reperfusion. The preservation of the hepatic function was evaluated by determining mitochondrial swelling and malondialdehyde tissue level, as well as alanine aminotransferase and aspartate aminotranferase serum levels.

Analysis of 83 consecutive liver transplants performed at a tertiary care reference hospital in the interior of the state of Sao Paulo.

Purpose: To analyze pre-, intra- and immediate postoperative parameters of patients submitted to liver transplantation.

Methods: Eighty-three consecutive orthotopic liver transplants performed from January 2009 to July 2011 were analyzed. The patients were divided into 2 groups: A, survivors (MELD between 9 and 60) and B, non-survivors (MELD between 14 and 40), with 30.

Exhaled breath condensate collection for nitrite dosage: a safe and low cost adaptation.

Purpose: Standardization of a simple and low cost technique of exhaled breath condensate (EBC) collection to measure nitrite.

Methods: Two devices were mounted in polystyrene boxes filled either with crushed ice/salt crystals or dry ice/crushed ice. Blood samples were stored at -70 degrees C for posterior nitrite dosages by chemiluminescence and the Griess reaction.

Will use of exhaled breath condensate be useful for the intensive care unit routine?

Endogenous production of nitric oxide can be detected and monitored in exhaled air of men and animals. The main objective of this review was to discuss if nitric oxide in exhaled breath condensate is a useful tool when investigating respiratory dysfunctions in intensive care units. Specialized literature reveals an increasing interest in the use of exhaled breath condensate as a non-invasive method to investigate pulmonary disease.

Proliferative effect of the aqueous extract of Hyptis pectinata on liver regeneration after partial hepatectomy in rats.

Purpose: This study was carried out to assess the effects of the aqueous extract of Hyptis pectinata leaves on liver regeneration and on serum enzymes (AST, ALT and gamma-GT) after 67% partial hepatectomy in rats.

Methods: AST, ALT and gamma-GT, were determined by conventional procedures using a spectrophotometer (Model E2250-CELM). Liver regeneration was evaluated by immunohistochemical staining for proliferating cell nuclear antigen (PCNA).

Pyruvate kinase activation and lipoperoxidation after selective hepatic ischemia in Wistar rats.

Purpose: Hepatic ischemia and reperfusion can cause several problems in hepatic surgery. The aim of this study was to determine pyruvate kinase activation and lipid peroxidation after hepatic ischemia.

Methods: Twenty-four Wistar rats were submitted to 90 minutes of selective liver ischemia and 15 minutes of reperfusion.