Effects of hyperbaric oxygen (HBO), as pre-conditioning in liver of rats submitted to periodic liver ischemia/reperfusion.

Purpose: to assess the effect of hyperbaric oxygen (HBO) as pre-conditioning on periodic liver ischemia/reperfusion injury.

Methods: Thirty-six male Wistar rats were divided into 4 groups (SHAM, I/R , HBO-I/R and CONTROL). The surgical technique consisted of total clamping of the hepatic pedicle for 15 min followed by twice repeated reperfusion for 5 min (unclamping).

Effect of hyperbaric oxygen therapy on liver function during intermittent ischemia.

Purpose: To analyze the effects of hyperbaric oxygen therapy on liver function in rats previously subjected to ischemia and reperfusion.

Methods: A randomly distribution of 23 Wistar rats was conducted into three groups: SHAM, animals subjected to surgical stress without restricting blood flow by clamping the hepatic pedicle, IR, rats underwent hepatic vascular occlusion intermittently for two complete cycles of 15 minutes of ischemia followed by 5 min of reperfusion, IR / HBO, rats underwent hepatic pedicle clamping and thereafter exposed to hyperbaric oxygen pressure of 2 absolute atmospheres for 60 minutes. We evaluated liver function through mitochondrial function, determined by the stages 3 and 4 of respiration, respiratory control ratio (RCR) and mitochondrial permeability transition (Swelling).

Biochemical liver function after partial hepatic resection with or without partial hepatic vascular exclusion.

Purpose: The aim of the present study was to assess the advantages and disadvantages of liver vascular partial exclusion (LVPE) (liver dysfunction due to ischemia) during liver resection in patients submitted to partial hepatectomy.

Methods: A total of 114 patients were submitted to partial hepatectomy (minor versus major resections) with LPVE being used in 57 of them but not in the remaining 57. Patient age ranged from 35 to 73 years and 57 % were women.

The influence of hemorrhagic shock on rat liver regeneration after partial hepatectomy: serum aminotranspherases, mitochondrial function, and hepatocellular replication studies.

This study was designed to evaluate the influence of hemorrhagic shock on hepatic regeneration in rats submitted to partial hepatectomy. The experimental protocol included 26 male Wistar rats, randomly assigned to 4 groups: GI: simulated operation; GII: 30% hepatectomy without hemorrhagic shock; GIII: only hemorrhagic shock; GIV: 30% hepatectomy associated with hemorrhagic shock. The methodologies used were: determination of aminotranspherases plasma levels; analysis of mitochondrial respiration, membrane potential and osmotic swelling; and markers of hepatocellular replication.