Publications by authors named "Maria Dolores Lozano-Arana"
Article Synopsis
- * In a study involving 35 couples since 2010, results showed a 64.4% transfer rate and an 18.6% live birth rate in the PGD program, highlighting lower clinical success for couples with affected females compared to those with affected males or different conditions.
- * The study found that women with DM1 had significantly lower percentages of mature oocytes and fertilization rates, suggesting poorer PGD outcomes for these women, though the exact reasons for
From 106 human blastocyts donate for research after in vitro fertilization (IVF) and preimplantation genetic diagnosis (PGD) for monogenetic disorder, 3 human embryonic stem cells (hESCs) HVR1, HVR2 and HVR3 were successfully derived. HVR1 was assumed to be genetically normal, HVR2 carrying Becker muscular dystrophy and HVR3 Hemophilia B. Despite the translocation t(9;15)(q34.
View Article and Find Full Text PDFFragile X syndrome (FXS) accounts for about one-half of cases of X-linked intellectual disability and is the most common monogenic cause of mental impairment. Reproductive options for the FXS carriers include preimplantation genetic diagnosis (PGD). However, this strategy is considered by some centers as wasteful owing to the high prevalence of premature ovarian failure in FXS carriers and the difficulties in genetic diagnosis of the embryos.
View Article and Find Full Text PDFHemophilia A and B are the most common hereditary hemorrhagic disorders, with an X-linked mode of inheritance. Reproductive options for the families affected with hemophilia, aiming at the prevention of the birth of children with severe coagulation disorders, include preimplantation genetic diagnosis (PGD). Here we present the results of our PGD Program applied to hemophilia, at the Department of Genetics, Reproduction and Fetal Medicine of the University Hospital Virgen del Rocío in Seville.
View Article and Find Full Text PDFArticle Synopsis
- Preimplantation genetic diagnosis with HLA matching (PGD-HLA) allows couples at risk of passing on genetic diseases to select healthy embryos that match the tissue type of their affected child.
- A study from the University Hospital Virgen del Rocío in Seville involved 7 couples, resulting in 26 cycles and 202 embryos, achieving a 96% success rate in diagnosing the embryos' genetic status and HLA-typing.
- The program led to the birth of 2 healthy babies who matched their affected siblings genetically, both of whom have successfully undergone stem cell transplants and are doing well months later.
Preimplantation genetic diagnosis (PGD) of single gene disorders, combined with HLA matching (PGD-HLA), has emerged as a tool for couples at risk of transmitting a genetic disease to select unaffected embryos of an HLA tissue type compatible with that of an existing affected child. Here, we present a novel one-step multiplex PCR to genotype a spectrum of STRs to simultaneously perform HLA typing and PGD for β-thalassemia. This method is being routinely used for PGD-HLA cycles in our department, with a genotyping success rate of 100%.
View Article and Find Full Text PDFObjective: To develop a multiplex polymerase chain reaction (PCR) method for Huntington disease (HD) preimplantation genetic diagnosis (PGD) based on the coamplification of CAG repeats and three different polymorphic microsatellites in a single step of PCR.
Design: Techniques and instrumentation.
Setting: Tertiary clinical and academic medical center.
Objective: To report a case of quadruple gestation (two sets of monozygotic twins) after intracytoplasmic sperm injection (ICSI) and transfer of two embryos.
Design: Case report.
Setting: Tertiary clinical and academic medical center.