Pyriproxyfen Contamination in : Identifying Early Warning Biomarkers.

Pyriproxyfen is an insecticide currently employed in numerous countries for the management of agricultural and indoor pests. Several studies indicate that this insecticide has been detected in multiple rivers, with concentrations reaching as high as 99.59 ng/L in the Júcar River in Spain.

Gene expression study alerted to possible impairment in Daphnia magna individuals as a consequence of exposure to sublethal concentrations of prochloraz.

In the present study, Daphnia magna individuals were exposed for 21 days to 87, 130, 170, 230 and 380 μg/L of prochloraz. The expression of genes related to lipid metabolism (fabd), oxidative stress (cat and gst), heat shock proteins synthesis (hsp70 and hsp90), haemoglobin synthesis (hgb1 and hgb2), metallothioneins synthesis (mt-a, mt-b and mt-c), and vitellogenin synthesis (vgt1 y vgt2) were analyzed. Results showed that some gene expression in D.

The prochloraz chronic exposure to Daphnia magna derived in biochemical alterations of F0 generation daphnids and malformed F1 progeny.

In the present study, D. magna individuals were exposed to several sublethal prochloraz concentrations (87, 130, 170, 230 and 380 μg/L) for 21 days according to; the previous acute toxicity results. The fungicide effects on reproduction, survival, individual size, and growth population rate were evaluated after an exposure of 21 days, and no changes were observed compared to the control group.