Monomorphic epitheliotropic intestinal T-cell lymphoma (MEITL) complicated with intestinal obstruction and perforation.

We report the case of a patient who comes to the emergency department with abdominal pain, oral intolerance and bloody stools, being diagnosed with intestinal obstruction secondary to a jejunal tumor with contained perforation. A laparotomy was performed with resection of the jejunal tumor and taking biopsies from lesions with similar characteristics in the rest of the small bowel compatible with monomorphic epitheliotropic intestinal T-cell lymphoma (MEITL). The monomorphic epitheliotropic intestinal T-cell lymphoma is a rare intestinal tumor with a poor prognosis that is characterized by the proliferation of intraepithelial lymphocytes.

Europium(III)-Doped Gadolinium(III) Complex for High-Sensitivity Temperature Sensing in the Physiological Range.

A new Eu-doped Gd complex of formula [EuGd(pta)me-phen] was synthesized and structurally characterized (Hpta = benzoyltrifluoroacetone, me-phen = 5-methyl-1,10-phenanthroline). The photoluminescence study revealed that when the compound was excited at RT, under a 457 nm continuous laser, the material exhibited high luminescence due to the antenna effect of the ligands, as well as a good balance between the phosphorescence from the spin-forbidden triplet (from the organic ligands), and the characteristic lanthanide f-f transitions. The ratio between the previous emissions drastically changed when the sample was heated up to 62 °C inside a tubular furnace.

Autochthonous transmission of the Indomalayan parasite, Transversotrema patialense, in the Caribbean: Molecular, morphological, and experimental evidence.

The Asian freshwater snail Melanoides tuberculata has been established since the 1960s in the Americas, where it transmits cercariae of a small number of digenetic trematode species from its native range. In 2021-2022, 24 M. tuberculata were discovered shedding transversotrematid cercariae in Puerto Rico, where parasites of this snail have not been previously studied.

Compact Microfluidic Platform with LED Light-Actuated Valves for Enzyme-Linked Immunosorbent Assay Automation.

Lab-on-a-chip devices incorporating valves and pumps can perform complex assays involving multiple reagents. However, the instruments used to drive these chips are complex and bulky. In this article, a new wax valve design that uses light from a light emitting diode (LED) for both opening and closing is reported.

Advances in quantum dots as diagnostic tools.

Quantum dots (QDs) are crystalline inorganic semiconductor nanoparticles a few nanometers in size that possess unique optical electronic properties vs those of larger materials. For example, QDs usually exhibit a strong and long-lived photoluminescence emission, a feature dependent on size, shape and composition. These special optoelectronic properties make them a promising alternative to conventional luminescent dyes as optical labels in biomedical applications including biomarker quantification, biomolecule targeting and molecular imaging.

Strip modification and alternative architectures for signal amplification in nanoparticle-based lateral flow assays.

Nanoparticle (NP)-based lateral flow assay (LFA) technology has outstanding characteristics that make it ideal for point-of-care bioanalytical applications. However, LFAs still have important limitations, especially related to sensitivity, which is in general worse than that of other well-established bioassays such as ELISA or PCR. Many efforts have been made for enhancing the sensitivity of LFAs, mainly actuating on the nanoparticle labels and on alternative optical detection modes.

Quantum Dot Bioconjugates for Diagnostic Applications.

Quantum dots (QDs) are a special type of engineered nanomaterials with outstanding optoelectronic properties that make them as a very promising alternative to conventional luminescent dyes in biomedical applications, including biomolecule (BM) targeting, luminescence imaging and drug delivery. A key parameter to ensure successful biomedical applications of QDs is the appropriate surface modification, i.e.

Bioelectrochromic hydrogel for fast antibiotic-susceptibility testing.

Materials science offers new perspectives in the clinical analysis of antimicrobial sensitivity. However, a biomaterial with the capacity to respond to living bacteria has not been developed to date. We present an electrochromic iron(III)-complexed alginate hydrogel sensitive to bacterial metabolism, here applied to fast antibiotic-susceptibility determination.

Aqueous Exfoliation of Transition Metal Dichalcogenides Assisted by DNA/RNA Nucleotides: Catalytically Active and Biocompatible Nanosheets Stabilized by Acid-Base Interactions.

The exfoliation and colloidal stabilization of layered transition metal dichalcogenides (TMDs) in an aqueous medium using functional biomolecules as dispersing agents have a number of potential benefits toward the production and practical use of the corresponding two-dimensional materials, but such a strategy has so far remained underexplored. Here, we report that DNA and RNA nucleotides are highly efficient dispersants in the preparation of stable aqueous suspensions of MoS and other TMD nanosheets at significant concentrations (up to 5-10 mg mL). Unlike the case of common surfactants, for which adsorption on 2D materials is generally based on weak dispersive forces, the exceptional colloidal stability of the TMD flakes was shown to rely on the presence of relatively strong, specific interactions of Lewis acid-base type between the DNA/RNA nucleotide molecules and the flakes.

Multiple actuation microvalves in wax microfluidics.

Microvalves are an essential component of microfluidic devices. In this work, a low-consumption (<35 mJ), fast-response (<0.3 s), small footprint (<0.

A microfluidic device for the automated electrical readout of low-density glass-slide microarrays.

Microarrays are a powerful platform for rapid and multiplexed analysis in a wide range of research fields. Electrical readout systems have emerged as an alternative to conventional optical methods for microarray analysis thanks to its potential advantages like low-cost, low-power and easy miniaturization of the required instrumentation. In this work an automated electrical readout system for low-cost glass-slide microarrays is described.

Microbial trench-based optofluidic system for reagentless determination of phenolic compounds.

Phenolic compounds are one of the main contaminants of soil and water due to their toxicity and persistence in the natural environment. Their presence is commonly determined with bulky and expensive instrumentation (e.g.

Reusable conductimetric array of interdigitated microelectrodes for the readout of low-density microarrays.

Low-density protein microarrays are emerging tools in diagnostics whose deployment could be primarily limited by the cost of fluorescence detection schemes. This paper describes an electrical readout system of microarrays comprising an array of gold interdigitated microelectrodes and an array of polydimethylsiloxane microwells, which enabled multiplexed detection of up to thirty six biological events on the same substrate. Similarly to fluorescent readout counterparts, the microarray can be developed on disposable glass slide substrates.

Fabrication of biofunctionalized microfluidic structures by low-temperature wax bonding.

In this work, a new fabrication technology for microfluidics based on the use of wax is described. Microfluidic structures are assembled using wax as both a thermoplastic adhesive layer between two glass substrates and a spacer layer defining the microchannels. Wax patterns with dimensions down to 25 μm are easily produced on glass substrates using specially developed decal-transfer microlithography.

Phenolic compounds as enhancers in enzymatic and electrochemical oxidation of veratryl alcohol and lignins.

Sixteen phenolic compounds, 14 of which naturally occurring, were compared to the synthetic 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and violuric acid (VA) in terms of their ability to act as mediators/enhancers in: (1) laccase oxidation of veratryl alcohol as a lignin model compound, and (2) electrochemical oxidation of kraft and flax lignins. HPLC analysis revealed that the syringyl-type phenols methyl syringate and acetosyringone were the most efficient natural enhancers in the laccase oxidation of veratryl alcohol. Both compounds, though far from the performance of ABTS were able to generate veratraldehyde in amount similar to that obtained with VA.

Multifunctional modification of wool using an enzymatic process in aqueous-organic media.

An enzymatic method using laccases for grafting the water insoluble phenolic compound lauryl gallate on wool fabric was developed. To find the compromise conditions at which the substrate is soluble while the enzyme remains active, the reaction was carried out in an 80/20 (v/v, %) aqueous-ethanol mixture, where the enzyme retains 75-80% of its activity. The enzymatic coating of wool with lauryl gallate provided in a one-step process a multifunctional textile material with antioxidant, antibacterial and water repellent properties.

Development of an immunosensor for the determination of rabbit IgG using streptavidin modified screen-printed carbon electrodes.

Voltammetric enzyme immunosensors based on the employment of streptavidin modified screen-printed carbon electrodes (SPCEs) for the detection of rabbit IgG, as a model analyte, were described. Alkaline phosphatase (AP) and 3-indoxyl phosphate (3-IP) were used as the enzymatic label and substrate, respectively. The adsorption of streptavidin was performed by deposition of a drop of a streptavidin solution overnight at 4 degrees C on the pre-oxidized surface of the SPCEs.

DNA hybridization biosensors using polylysine modified SPCEs.

Two electrochemical DNA hybridization biosensors (genosensors) for the detection of a 30-mer sequence unique to severe acute respiratory syndrome (SARS) virus are described in this work. Both genosensors rely on the hybridization of the oligonucleotide target with its complementary probe, which is immobilized on positively charged polylysine modified screen-printed carbon electrodes (SPCEs), through electrostatic interactions. In one design, a biotinylated target is used and the detection of the hybridization reaction is monitored using alkaline phosphatase labeled streptavidin (S-AP).

Immunosensor for Mycobacterium tuberculosis on screen-printed carbon electrodes.

In this work, two methods have been compared to produce enzymatic voltammetric immunosensors for the determination of Mycobacterium tuberculosis antigens (Ag360 and Ag231), using a pre-oxidised screen-printed carbon electrode (SPCE) as a signal transduction element. The enzyme alkaline phosphatase (AP) was used in combination with the substrate 3-indoxyl phosphate (3-IP). In one design, the immune complexes between M.

Enzymatic genosensor on streptavidin-modified screen-printed carbon electrodes.

Voltammetric enzyme genosensors on streptavidin-modified screen-printed carbon electrodes (SPCEs) for the detection of virulence nucleic acid determinants of pneumolysin and autolysin genes, exclusively present on the genome of the human pathogen Streptococcus pneumoniae, were described. Alkaline phosphatase (AP) and 3-indoxyl phosphate were used as the enzymatic label and substrate, respectively. The oligonucleotide probes were immobilized on electrochemically pretreated SPCEs through the streptavidin/biotin reaction.

Indirect determination of alkaline phosphatase based on the amperometric detection of indigo carmine at a screen-printed electrode in a flow system.

Amperometric analysis of indigo carmine at a bare screen-printed electrode placed in an FIA system is reported. This compound is easily detected at a potential of -0.3 V (vs.