Outer membrane vesicles (OMVs) from Gram-negative bacteria were first described more than 50 years ago. However, the molecular mechanisms involved in biogenesis began to be studied only in the last few decades. Presently, the biogenesis and molecular mechanisms for their release are not completely known.
View Article and Find Full Text PDFGram-negative bacteria release nanovesicles, called outer membrane vesicles (OMVs), from their outer membrane. Proteomics has been used to determine their composition. OMVs contain proteins able to elicit an immune response, so they have been proposed as a model to develop acellular vaccines.
View Article and Find Full Text PDFMembrane blebs are released from Gram-negative bacteria, however, little is known about blebs. This work pursued two objectives, the first was to determine and identify the proteins in the membrane blebs by proteomics and analysis. The second aim was to evaluate the use of membrane blebs of 2308 and RB51 as an acellular vaccine and .
View Article and Find Full Text PDFGram-negative bacteria release outer membrane vesicles (OMVs) into the extracellular environment. OMVs have been studied extensively in bacterial pathogens, however, information related with the composition of OMVs is missing. In this study we analyzed the composition of purified OMVs from ATCC 7966 by proteomics.
View Article and Find Full Text PDFThe present report describes the misidentification of spp. from a positive blood culture using traditional microbiology tests. A molecular test identified the bacterium as .
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