Evaluation of the influence of the gas residence time and biomass concentration on methane bioconversion to ectoines in a novel Taylor flow bioreactor.

Today, the use of biogas to produce more sustainable bioproducts is attracting an increasing attention in the quest for a circular economy. This work aims at optimizing the biosynthesis of high value bioproducts such as ectoine and hydroxyectoine from methane using a high mass transfer Taylor flow reactor and a methanotrophic consortium. The influence of the gas residence time (30-240 min) and concentration of microorganisms (0.

Optimization of methane gas-liquid mass transfer during biogas-based ectoine production in bubble column bioreactors.

Nowadays, the utilization of biogas for energy generation is hindered by the declining production costs of solar and wind power. A shift towards the valorization of biogas into ectoine, a highly valuable bioproduct priced at 1000 €⸱kg, offers a novel approach to fostering a more competitive biogas market while contributing to carbon neutrality. This study evaluated the optimization of CH gas-liquid mass transfer in 10 L bubble column bioreactors for CH conversion into ectoine and hydroxyectoine using a mixed methanotrophic culture.

Ectoines production from biogas in pilot bubble column bioreactors and their subsequent extraction via bio-milking.

Despite the potential of biogas from waste/wastewater treatment as a renewable energy source, the presence of pollutants and the rapid decrease in the levelized cost of solar and wind power constrain the use of biogas for energy generation. Biogas conversion into ectoine, one of the most valuable bioproducts (1000 €/kg), constitutes a new strategy to promote a competitive biogas market. The potential for a stand-alone 20 L bubble column bioreactor operating at 6% NaCl and two 10 L interconnected bioreactors (at 0 and 6% NaCl, respectively) for ectoine production from biogas was comparatively assessed.

Influence of operational conditions on the performance of biogas bioconversion into ectoines in pilot bubble column bioreactors.

The application of biogas as a low-priced substrate for the production of ectoines constitutes an opportunity to decrease their production costs and to enhance the viability of anaerobic digestion. The influence of operational conditions on CH-biogas biodegradation and on ectoines production yields was assessed in continuous pilot bubble column bioreactors. The rise in biomass concentration from 1 to 3 g L resulted in a decrease in the specific ectoine content from 42 ± 8 to 30 ± 4 mg g.

Performance evaluation of a control strategy for photosynthetic biogas upgrading in a semi-industrial scale photobioreactor.

The validation of a control strategy for biogas upgrading via light-driven CO consumption by microalgae and HS oxidation by oxidizing bacteria using the oxygen photosynthetically generated was performed in a semi-industrial scale (9.6 m) photobioreactor. The control system was able to support CO concentrations lower than 2% with O contents ≤ 1% regardless of the pH in the cultivation broth (ranging from 9.

Technology validation of photosynthetic biogas upgrading in a semi-industrial scale algal-bacterial photobioreactor.

The performance of photosynthetic biogas upgrading coupled to wastewater treatment was evaluated in an outdoors high rate algal pond (HRAP) interconnected to an absorption column at semi-industrial scale. The influence of biogas flowrate (274, 370 and 459 L h), liquid to biogas ratio (L/G = 1.2, 2.