Cannabinoid Receptor 1 associates to different molecular complexes during GABAergic neuron maturation.

CB cannabinoid receptor is widely expressed in the central nervous system of animals from late prenatal development to adulthood. Appropriate activation and signaling of CB cannabinoid receptors in cortical interneurons are crucial during perinatal/postnatal ages and adolescence, when long-lasting changes in brain activity may elicit subsequent appearance of disorders in the adult brain. Here we used an optimized immunoprecipitation protocol based on specific antibodies followed by shot-gun proteomics to find CB interacting partners in postnatal rat GABAergic cortical neurons in vitro at two different stages of maturation.