In this work, three isolates of Plasmodium juxtanucleare have been analyzed based on morphological, morphometric and parasitic parameters. Each isolate was sampled from naturally infected adult chicken (Gallus gallus) from rural areas of three Brazilian municipalities: Seropédica (22 degrees 48' S; 43 degrees 41' W), in the state of Rio de Janeiro; Cruzeiro (22 degrees 33' S; 44 degrees 57' W), in the state of São Paulo; and Santa Bárbara do Tugúrio (21 degrees 15' S; 43 degrees 27' W), in the state of Minas Gerais. The blood samples taken from each infected chicken were inoculated in three groups of ten young chicken (21 days old).
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