Soybean Embryonic Axis Transformation: Combining Biolistic and -Mediated Protocols to Overcome Typical Complications of Plant Regeneration.

The first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing mediated genetic transformation. Limitations over infectivity and tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods.