Quantification methods of are independent irrespective of fungal morphology.

The ability of to switch its morphology from yeast to filaments, known as polymorphism, may bias the methods used in microbial quantification. Here, we compared the quantification methods [cell/mL, colony forming units (CFU)/mL, and the number of nuclei estimated by viability polymerase chain reaction (vPCR)] of three strains of (one reference strain and two clinical isolates) grown as yeast, filaments, and biofilms. Metabolic activity (XTT assay) was also used for biofilms.

Verapamil inhibits efflux pumps in , exhibits synergism with fluconazole, and increases survival of .

The emergence of resistance requires alternative methods to treat infections. We evaluated efficacy of the efflux pump inhibitor (EPI) verapamil (VER) with fluconazole (FLC) against FLC-resistant (CaR) and -susceptible (CaS). The susceptibility of both strains to VER and FLC was determined, as well as the synergism of VER with FLC.

Sugarcane biopolymer patch in femoral vein angioplasty on dogs.

Purpose: To evaluate the use of the sugarcane biopolymer membrane in femoral vein patch angioplasty on dogs.

Methods: Eight dogs were submitted to bilateral femoral vein patch angioplasty with a sugarcane biopolymer membrane patch on one side and an expanded polytetrafluoroethylene (e-PTFE) patch on the contralateral side. This research was performed at Experimental Surgical Research Laboratory of the Centro de Ciências da Saúde at Universidade Federal de Pernambuco.

Adenoviruses isolated from civilian and military personnel in the city of Rio de Janeiro, Brazil.

Adenovirus are important pathogen primarily associated to respiratory infections of children and military personnel, even though it is also associated to cases of conjunctivitis and keratoconjunctivitis. We analyzed respiratory secretion collected from subjects with and without respiratory infection symptoms, being 181 civilians and 221 military subjects. The samples were inoculated in HEp-2 and/or A549 tissue cultures for viral isolation.