This study demonstrates a simple approach to synthesize green Cu particles stabilized by poly(n-vinyl)pyrrolidone (PVP): the latter acts as stabilizer and dispersant, and its presence in solution eliminates the need for an inert atmosphere. Synthetic parameters were tuned to obtain particles with diameters >200 nm, to be human-safe and prevent nano-cytotoxicity. PVP and reductant concentrations, with reaction times, were varied to investigate their effect on colloidal stability, kinetics, and particles size.
View Article and Find Full Text PDFIn this work, we demonstrate that a simple argentometric titration is a scalable, fast, green and robust approach for producing AgCl/antibiotic hybrid antimicrobial materials. We titrated AgNO into tetracycline hydrochloride (TCH) aqueous solution, thus forming AgCl/TCH in a one-step procedure. Furthermore, we investigated the one-pot synthesis of triply synergistic super-nanoantimicrobials, combining an inorganic source of Ag ions (AgCl), a disinfecting agent (benzyl-dimethyl-hexadecyl-ammonium chloride, BAC) and a molecular antibiotic (tetracycline hydrochloride, TCH).
View Article and Find Full Text PDFOur society largely relies on inorganic semiconductor devices which are, so far, fabricated using expensive and complex processes requiring ultra-high vacuum equipment. Here we report on the possibility of growing a p-n junction taking advantage of electrochemical processes based on the use of aqueous solutions. The growth of the junction has been carried out using the Electrochemical Atomic Layer Deposition (E-ALD) technique, which allowed to sequentially deposit two different semiconductors, CdS and CuS, on an Ag(111) substrate, in a single procedure.
View Article and Find Full Text PDFOne of the crucial challenges of our time is to effectively use metal and metal oxide nanoparticles (NPs) as an alternative way to combat drug-resistant infections. Metal and metal oxide NPs such as Ag, AgO, Cu, CuO, CuO, and ZnO have found their way against antimicrobial resistance. However, they also suffer from several limitations ranging from toxicity issues to resistance mechanisms by complex structures of bacterial communities, so-called biofilms.
View Article and Find Full Text PDFUnlike other antimicrobial agents, Ag-based composites are stable and currently widely used as broad spectral additives, fighting microbial biofilms and other biological threats. The goal of the present study is to develop a green, multifunctional, and robust antibiofilm water-insoluble coating, inhibiting histamine-producing biofilms. Herein, laser-ablated Ag NPs (L-Ag NPs) were incorporated into and onto a montmorillonite (MMT) surface layer with a simple wet chemical method, provided that the electrostatic interaction between L-Ag NPs and MMT clay led to the formation of L-Ag/MMT nanoantimicrobials (NAMs).
View Article and Find Full Text PDFThis study demonstrates a simple and reproducible approach to synthesize green core-shell copper sub-microparticles stabilized by poly(n-vinyl)pyrrolidone (PVP). Cu@PVP colloids were here prepared using copper sulfate pentahydrate as precursor and glucose as reducing agent. The presence of PVP in the synthetic medium eliminates the need for an inert atmosphere during the process, thus simplifying the whole method.
View Article and Find Full Text PDFThin self-standing films with potential antimicrobial synergistic activity have been produced by a simple green chemical synthesis with overnight thermal treatment. Their properties have been studied by scanning electron microscopy, X-ray photoelectron spectroscopy and other techniques to understand their potential range of applications. In this work, the focus was set on the development of a potential novel and effective alternative to conventional antimicrobial materials.
View Article and Find Full Text PDFFoodborne pathogenic microorganisms form biofilms at abiotic surfaces, which is a particular challenge in food processing industries. The complexity of biofilm formation requires a fundamental understanding on the involved molecular mechanisms, which may then lead to efficient prevention strategies. In the present study, biogenic amine producing bacteria, i.
View Article and Find Full Text PDFAll over the world, one of the major challenges is the green synthesis of potential materials against antimicrobial resistance and viruses. This study demonstrates a simple method like chemistry lab titration to synthesize green, facile, scalable, reproducible, and stable synergistic silver chloride/benzyldimethylhexadecyl-ammonium chloride (AgCl/BAC) colloidal Nanoantimicrobials (NAMs). Nanocolloidal dispersions of AgCl in an aqueous medium are prepared by using silver nitrate (AgNO) as precursor and BAC as both sources of chloride and stabilizer, holding an asymmetric molecular structure.
View Article and Find Full Text PDFSilver-fluoropolymer (Ag-CF) composed of encapsulated bioactive nanophases within a thin polymer coating are promising antimicrobial films with excellent bioactivity. In this contribution, we report on Ag-CF thin films obtained by ion beam co-sputtering, accurately tuning film thickness, and inorganic loading. The Ag-CF films were characterized by spectroscopic and scanning probe microscopy techniques with respect to composition and swelling behavior.
View Article and Find Full Text PDFIn 2019, the new coronavirus disease (COVID-19), related to the severe acute respiratory syndrome coronavirus (SARS-CoV-2), started spreading around the word, giving rise to the world pandemic we are still facing. Since then, many strategies for the prevention and control of COVID-19 have been studied and implemented. In addition to pharmacological treatments and vaccines, it is mandatory to ensure the cleaning and disinfection of the skin and inanimate surfaces, especially in those contexts where the contagion could spread quickly, such as hospitals and clinical laboratories, schools, transport, and public places in general.
View Article and Find Full Text PDFBiofilms are a major cause of health and environmental issues. Bacteria organized in biofilms are much more resistant to biocides than their equivalents in the planktonic state. In this context, spectroscopic techniques have significantly contributed to a more fundamental understanding of biofilm formation, which is crucial to prevent and limit their generation, spreading, and maturation.
View Article and Find Full Text PDFRecently nanoparticle enhanced Laser Induced Breakdown Spectroscopy (NELIBS) is getting a growing interest as an effective alternative method for improving the analytical performance of LIBS. On the other hand, the plasmonic effect during laser ablation can be used for a different task rather than elemental analysis. In this paper, the dependence of NELIBS emission signal enhancement on nanoparticle-protein solutions dried on a reference substrate (metallic titanium) was investigated.
View Article and Find Full Text PDFDesigning bioactive materials, with controlled metal ion release, exerting a significant biological action and associated to low toxicity for humans, is nowadays one of the most important challenges for our community. The most looked-for nanoantimicrobials are capable of releasing metal species with defined kinetic profiles, either by slowing down or inhibiting bacterial growth and pathogenic microorganism diffusion. In this study, laser ablation synthesis in solution (LASiS) has been used to produce bioactive Ag-based nanocolloids, in isopropyl alcohol, which can be used as water-insoluble nano-reservoirs in composite materials like poly(3-hydroxybutyrate-co-3-hydroxyvalerate).
View Article and Find Full Text PDFThe emerging problem of the antibiotic resistance development and the consequences that the health, food and other sectors face stimulate researchers to find safe and effective alternative methods to fight antimicrobial resistance (AMR) and biofilm formation. One of the most promising and efficient groups of materials known for robust antimicrobial performance is noble metal nanoparticles. Notably, silver nanoparticles (AgNPs) have been already widely investigated and applied as antimicrobial agents.
View Article and Find Full Text PDFGold nanoparticles (AuNPs) were produced using stainless steel as a solid reductant to assist the synthesis of metal NPs, using HAuCl as a precursor. This method is very easy, quick, and cost-effective, allowing the synthesis of highly stable NPs without additional capping agents. However, the reaction mechanism is still under debate.
View Article and Find Full Text PDFZinc oxide (ZnO) nanostructures are widely applied materials, and are also capable of antimicrobial action. They can be obtained by several methods, which include physical and chemical approaches. Considering the recent rise of green and low-cost synthetic routes for nanomaterial development, electrochemical techniques represent a valid alternative to biogenic synthesis.
View Article and Find Full Text PDFThe rapid spreading of resistance among common bacterial pathogens towards the misused antibiotics/disinfectant agents has drawn much attention worldwide to bacterial infections. In light of this, the present work aimed at the realization of core-shell nanoparticles possessing remarkable antimicrobial properties thanks to the synergistic action of the metal core and the disinfectant shell. Copper nanoparticles stabilized by benzalkonium chloride were prepared, characterized, and implemented in poly-vinyl-methyl ketone to obtain nanoantimicrobial composite coatings.
View Article and Find Full Text PDFSilver nanoparticles (AgNPs) are well-known for their antimicrobial effects and several groups are proposing them as active agents to fight antimicrobial resistance. A wide variety of methods is available for nanoparticle synthesis, affording a broad spectrum of chemical and physical properties. In this work, we report on AgNPs produced by laser ablation synthesis in solution (LASiS), discussing the major features of this approach.
View Article and Find Full Text PDFThe development of antibacterial coatings is of great interest from both industry and the consumer's point of view. In this study, we characterized tanned leather and polyurethane leatherette, typically employed in the automotive and footwear industries, which were modified by photo-deposition of antibacterial silver nanoparticles (AgNPs). Material surface chemical composition was investigated in detail by X-ray photoelectron spectroscopy (XPS).
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