Publications by authors named "Maria Berenice R Steffens"

A draft genome sequence of type strain CBS 980.96 was obtained. This species was first isolated from a cat with cerebral phaeohyphomycosis in Queensland, Australia.

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On the basis of multilocus phylogenetic data, Fonsecaea nubica was described in 2010 as a molecular sibling of F. monophora, an established agent of the human skin disease chomoblastomycosis in tropical zones. Genome analysis of these pathogens is mandatory to identify genes involved in the interaction with host and virulence.

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The black yeast Fonsecaea monophora is one of the main etiologic agents of chromoblastomycosis in humans. Its pathogenicity profile is more invasive than that of related Fonsecaea species, causing brain infection in addition to (sub)cutaneous infections.

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Article Synopsis
  • The study focuses on the interaction mechanisms between the endophytic bacterium Herbaspirillum seropedicae and plant hosts, revealing that its genetic makeup supports versatile colonization and nutrient exchange.
  • The genome of H. seropedicae, which has over 4,800 genes, indicates its ability to metabolize various carbon and nitrogen sources, as well as to produce hormones like indole acetic acid that can influence plant growth.
  • The presence of multiple protein secretion systems and adhesion-related genes suggests that H. seropedicae is well-equipped to interact with and thrive inside a diverse range of plant species.
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The Herbaspirillum seropedicae RecX protein participates in the SOS response: a process in which the RecA protein plays a central role. The RecX protein of the H. seropedicae, fused to a His-tag sequence (RecX His-tagged), was over-expressed in Escherichia coli and purified by metal-affinity chromatography to yield a highly purified and active protein.

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Abstract The interactions between maize, sorghum, wheat and rice plants and Herbaspirillum seropedicae were examined microscopically following inoculation with the H. seropedicae LR15 strain, a Nif(+) (Pnif::gusA) mutant obtained by the insertion of a gusA-kanamycin cassette into the nifH gene of the H. seropedicae wild-type strain.

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The recA and the recX genes of Herbaspirillum seropedicae were sequenced. The recX is located 359 bp downstream from recA. Sequence analysis indicated the presence of a putative operator site overlapping a probable sigma70-dependent promoter upstream of recA and a transcription terminator downstream from recX, with no apparent promoter sequence in the intergenic region.

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