Publications by authors named "Maria Azcona"
Article Synopsis
- Multiplex gene regulation allows for the simultaneous adjustment of multiple gene expressions to fine-tune complex cellular processes, often used alongside genome editing tools.
- In cancer immunotherapy, techniques like multiplex genome editing have been employed to edit three specific genes to create CAR T cells that resist tumor suppression.
- A safer alternative, epigenome editing, enables precise gene expression modification without causing DNA damage, and this protocol outlines how to silence specific genes in CAR T cells to enhance their effectiveness against tumors.
Article Synopsis
- The study investigated the prevalence of celiac disease (CD) in children across five urban districts in Argentina, as no prior data existed.
- Researchers analyzed serum samples from 2,219 children, aged 3-16, using serological tests to detect CD and proposed biopsies for further confirmation.
- The study found a prevalence of 1.26% with a notable female predominance, highlighting a need for increased awareness and early testing for celiac disease among pediatric populations to prevent complications.
Unlabelled: The identification of molecular mechanisms involved in the maintenance of the transformed phenotype of hepatocellular carcinoma (HCC) cells is essential for the elucidation of therapeutic strategies. Here, we show that human HCC cells display an autocrine loop mediated by connective tissue growth factor (CTGF) that promotes DNA synthesis and cell survival. Expression of CTGF was stimulated by epidermal growth factor receptor (EGFR) ligands and was dependent on the expression of the transcriptional coactivator, Yes-associated protein (YAP).
View Article and Find Full Text PDFHepatocarcinogenesis is a complex multistep process in which many different molecular pathways have been implicated. Hepatocellular carcinoma (HCC) is refractory to conventional chemotherapeutic agents, and the new targeted therapies are meeting with limited success. Interreceptor crosstalk and the positive feedback between different signaling systems are emerging as mechanisms of targeted therapy resistance.
View Article and Find Full Text PDFBiodegradable lactic acid-glycolic acid copolymer (PLGA) formulations incorporating cisplatin have been developed to evaluate the cytotoxicity of this agent in cultured cells. Two different W/O/W protocols were used to formulate micro- (MP) and nanoparticles (NP) under the solvent evaporation method. Although the amount of cisplatin encapsulated was higher in the MP, the efficiency of encapsulation was similar: 10.
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