[Comparison of two transportation media for study of normal individual conjunctival microbiota].

Purpose: To compare the microbiological profile of normal microbiota of healthy people obtained from conjunctival smear using dry swab in Stuart's transport medium and wet swab transported in test tube sealed with cotton.

Methods: A prospective study with random samples, performed at the Departments of Ophthalmology and Pathology of Santa Casa Misericórdia de São Paulo, in August of 2006. Eighty normal eyes of 40 healthy individuals were analyzed.

[Risk of contamination of trypan blue dye after first use].

Purpose: To determine the potential risk of contamination of a trypan blue bottle (TB) after first use and after being stored under different temperature and humidity conditions, as well as to identify possible contamination factors, most frequently involved microorganisms and simultaneously evaluate bacteriostatic and bactericide properties of the dye.

Methods: An experimental and prospective study was carried out, in which 30 TB bottles were divided into 3 groups (A: control, B: refrigerator storage and C: cabinet storage). The dye was suctioned and cultivated in agar blood plates and Sabouraud agar tube.