Background: Conventional cryopreservation methods induce chemical and mechanical damage to the sperm membranes. The cryoprotectant potential of phospholipids of vegetal origin as soybean lecithin has been investigated as a substitute for egg yolk in diluents used for the cryopreservation of human spermatozoa. Therefore, the objective of this study was comparing the efficacy of a synthetic cryoprotectant supplemented with L-α-phosphatidylcholine (PC) and L-acetyl-carnitine (ANTIOX-PC) and the standard egg-based TEST-yolk buffer (TYB) in preserving sperm motility and chromatin quality in cryopreserved semen samples.
View Article and Find Full Text PDFSyst Biol Reprod Med
April 2019
Varicocele pathophysiology is related to increased oxidative stress, which might result in loss sperm DNA integrity as well as in genomic instability. Sperm telomere shortening and loss of global DNA methylation are the main features of genomic instability, leading to cell senescence and death, whereas sperm DNA fragmentation (SDF) characterizes the loss of chromatin integrity. We hypothesize that sperm genomic stability and DNA integrity is reduced in infertile men with moderate and large-sized varicoceles, thus being candidate markers of sperm quality in varicocele-related infertility.
View Article and Find Full Text PDFObjectives: The objective of this research was to analyze the functional changes of lower limbs by means of surface electromyography in patients with acquired immunodeficiency syndrome.
Methods: A total of 60 men and women (age mean of 36.77 ± 9.
Purpose: To understand the effects of HIV type 1 on the function of the masticatory muscles.
Methods: Sixty individuals were selected from both genders, aged between 22 and 57years (mean 36.77±9.