Enteroaggregative as etiological agent of endemic diarrhea in Spain: A prospective multicenter prevalence study with molecular characterization of isolates.

Background: Enteroaggregative (EAEC) is increasingly associated with domestically acquired diarrheal episodes in high-income countries, particularly among children. However, its specific role in endemic diarrhea in this setting remains under-recognized and information on molecular characteristics of such EAEC strains is limited. We aimed to investigate the occurrence of EAEC in patients with non-travel related diarrhea in Spain and molecularly characterize EAEC strains associated with illness acquired in this high-income setting.

Mucus-Activatable Shiga Toxin Genotype stx2d in Escherichia coli O157:H7.

We identified the mucus-activatable Shiga toxin genotype stx2d in the most common hemolytic uremic syndrome-associated Escherichia coli serotype, O157:H7. stx2d was detected in a strain isolated from a 2-year-old boy with bloody diarrhea in Spain, and whole-genome sequencing was used to confirm and fully characterize the strain.

[Prevalence of Giardia duodenalis assemblage B in humans in Zaragoza and León, Spain].

Objectives: To study Giardia duodenalis assemblages circulating in the health areas of two hospitals in Zaragoza and León (Spain).

Methods: A total of 211 stool samples with Giardia were genotyped by PCR of the tpi gene.

Results: Assemblage B was the most prevalent, both in Zaragoza (84,7%) and León (95,1%).