Publications by authors named "Maria Ambilika"

() was developed as a microbial cell factory for the heterologous expression of fungal secondary metabolites. This was achieved by inactivation of sorbicillinoid biosynthesis and construction of vectors for the rapid cloning and expression of heterologous fungal biosynthetic genes. Two types of megasynth(et)ases were used to test the strain and vectors, namely a non-reducing polyketide synthase (nr-PKS, ) from and a hybrid highly-reducing PKS non-ribosomal peptide synthetase (hr-PKS-NRPS, ) from .

View Article and Find Full Text PDF

The biosynthetic gene cluster (BGC) from the well-studied organism was studied by heterologous expression in the fungal host . Coexpression of and produces two new acyl tetramic acids. Addition of the ring-expanding cytochrome P450 encoded by then yields a known pyridone intermediate to ilicicolin H and a new chain-truncated shunt metabolite.

View Article and Find Full Text PDF