Promising anticancer activity of batumin: a natural polyene antibiotic produced by Pseudomonas batumici.

Aim: To determine the computer-predicted anticancer activity of antibiotic batumin.

Materials & Methods: Cytotoxicity assays, cell morphology microscopy and cell cycle progression were studied in cancer and nontumorigenic cell lines. An in vivo experiment on Lewis lung carcinoma (3LL)-transplanted mice was conducted to evaluate potential antimetastatic.

Human beta-defensin 3 (hBD-3) expression in A431 cell line and human vulval tumors.

Unlabelled: The present work was AIMED on the study of human beta-defensin 3 (hBD-3) gene expression in A431 cell line and human vulval tumors.

Materials And Methods: Twenty surgical specimens of both malignant and conventionally normal human vulval tissues have been analyzed for the presence of hBD-3 mRNA by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). In vitro experiments have been carried out on A431 cells.