Noradrenaline-induced cation currents in isolated rat paratracheal ganglion neurons.

The actions of noradrenaline (NA) on the neurons acutely isolated from paratracheal ganglia of rats and the ionic mechanisms involved were studied with nystatin-perforated patch recording configuration. Under current-clamp conditions, application of 10 microM NA produced membrane depolarization followed by repetitive action potentials. NA evoked an inward cationic current under voltage-clamp conditions at a holding potential of -60 mV.

[The dissociation of paratracheal ganglion neurons and its application to neuropharmacology].

It is generally considered that dominant excitatory control of the airway is exerted by the parasympathetic nervous system. In the lower airway, there are a number of parasympathetic ganglia on the serosal surface of the dorsal tracheal wall. The dissociation of these paratracheal ganglion neurons facilitates the ability to visualize and patch-clamp single-neurons and to control the surrounding solutions.

Alpha 1-adrenoceptor-activated cation currents in neurones acutely isolated from rat cardiac parasympathetic ganglia.

The noradrenaline (NA)-induced cation current was investigated in neurones freshly isolated from rat cardiac parasympathetic ganglia using the nystatin-perforated patch recording configuration. Under current-clamp conditions, NA depolarized the membrane, eliciting repetitive action potentials. NA evoked an inward cation current under voltage-clamp conditions at a holding potential of -60 mV.