Genetic alterations in patients with chronic leucocytosis and persistent thrombocytosis.

To elucidate the relevance of genetic alterations, we analysed 17 genes known to be involved in haematological neoplasms in patients with chronic leucocytosis and patients with persistent thrombocytosis. Mutations of the , and genes were found in 1/13, 1/13, and 2/13 patients with leucocytosis, respectively. Mutations of the , , and genes were found in 1/5, 1/5, 1/5 and 2/5 patients with thrombocytosis, respectively.

Tumor suppressor gene methylation on the short arm of chromosome 1 in chronic myelogenous leukemia.

Objectives: We previously reported loss of heterozygosity on 1p in chronic myelogenous leukemia (CML). We analyzed promoter methylation and mutation of tumor suppressor genes on 1p36 in CML.

Methods: We performed methylation-specific PCR (MS-PCR) analysis of the PRDM2, RUNX3, and TP73 genes in 61 patients with CML (43 chronic phase, CP; two accelerated phase; and 16 blast crisis, BC).