Publications by authors named "Mari Nishii"

DNA immunization has been used to study vaccination methods and for production of specific antibodies. The present study aimed to apply DNA immunization to prepare specific IgYs, which react against rabies virus N protein (RV-N) and can be used to research and diagnose rabies virus. The DNA sequence of RV-N was ligated into a pcDNA 3.

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Article Synopsis
  • Atopic dermatitis (AD) is a common skin condition in kids that can worsen with specific bacterial colonization, and a specific egg yolk antibody (IgY) has been shown to inhibit this growth.
  • The study compared the effects of different adjuvants used in the immunization of hens to produce anti-IgY antibodies, noting that oil-based Freund's incomplete adjuvant (FIA) led to the most effective antibodies.
  • Among the purified antibodies, FIA-IgY demonstrated the strongest growth inhibition of bacteria, suggesting that oil-based adjuvants are crucial for developing IgY as a potential treatment for AD symptoms.
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We previously reported that a diet containing 65% paddy rice suppressed the colonization of in the cecum of broiler chicks, suggesting that this type of diet has positive effects on upper gastrointestinal tract function in broilers. To reveal the possible mechanisms involved in this antibacterial effect of the whole-grain paddy rice diet, we performed experiments comparing the digesta passage rate in the crop and gizzard, the development of gizzard, and the pH distribution in the gizzard between groups of chicks fed two different diets, such as ground corn and whole-grain paddy rice. During these experiments, we made the following observations: the chicks in the group fed the whole-grain paddy rice diet had more developed gizzards and significantly larger crop content than the chicks in the group fed the ground corn diet.

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