Validation of a 22-Gene Genomic Classifier in Patients With Recurrent Prostate Cancer: An Ancillary Study of the NRG/RTOG 9601 Randomized Clinical Trial.

Importance: Decipher (Decipher Biosciences Inc) is a genomic classifier (GC) developed to estimate the risk of distant metastasis (DM) after radical prostatectomy (RP) in patients with prostate cancer.

Objective: To validate the GC in the context of a randomized phase 3 trial.

Design, Setting, And Participants: This ancillary study used RP specimens from the phase 3 placebo-controlled NRG/RTOG 9601 randomized clinical trial conducted from March 1998 to March 2003.

Validation of the Decipher Test for Predicting Distant Metastatic Recurrence in Men with High-risk Nonmetastatic Prostate Cancer 10 Years After Surgery.

Background: Decipher is a genomic classifier designed to predict the development of distant metastases after surgical treatment of prostate cancer (PC). Its long-term prognostic role in a high-risk PC population has not been investigated previously.

Objective: To determine the prognostic role of the Decipher genomic classifier in two high-risk PC case-control studies.

The Diverse Genomic Landscape of Clinically Low-risk Prostate Cancer.

Background: Among men with clinically low-risk prostate cancer, we have previously documented heterogeneity in terms of clinical characteristics and genomic risk scores.

Objective: To further study the underlying tumor biology of this patient population, by interrogating broader patterns of gene expression among men with clinically low-risk tumors.

Design, Setting, And Participants: Prostate biopsies from 427 patients considered potentially suitable for active surveillance underwent central pathology review and genome-wide expression profiling.

Development and Validation of a Novel Integrated Clinical-Genomic Risk Group Classification for Localized Prostate Cancer.

Purpose It is clinically challenging to integrate genomic-classifier results that report a numeric risk of recurrence into treatment recommendations for localized prostate cancer, which are founded in the framework of risk groups. We aimed to develop a novel clinical-genomic risk grouping system that can readily be incorporated into treatment guidelines for localized prostate cancer. Materials and Methods Two multicenter cohorts (n = 991) were used for training and validation of the clinical-genomic risk groups, and two additional cohorts (n = 5,937) were used for reclassification analyses.

Ability of a Genomic Classifier to Predict Metastasis and Prostate Cancer-specific Mortality after Radiation or Surgery based on Needle Biopsy Specimens.

Background: Decipher is a validated genomic classifier developed to determine the biological potential for metastasis after radical prostatectomy (RP).

Objective: To evaluate the ability of biopsy Decipher to predict metastasis and Prostate cancer-specific mortality (PCSM) in primarily intermediate- to high-risk patients treated with RP or radiation therapy (RT).

Design, Setting, And Participants: Two hundred and thirty-five patients treated with either RP (n=105) or RT±androgen deprivation therapy (n=130) with available genomic expression profiles generated from diagnostic biopsy specimens from seven tertiary referral centers.

Sidewall carboxylic acid functionalization of single-walled carbon nanotubes.

The reactions of single-walled carbon nanotubes (SWNTs) with succinic or glutaric acid acyl peroxides in o-dichlorobenzene at 80-90 degrees C resulted in the addition of 2-carboxyethyl or 3-carboxypropyl groups, respectively, to the sidewalls of the SWNT. These acid-functionalized SWNTs were converted to acid chlorides by derivatization with SOCl(2) and then to amides with terminal diamines such as ethylenediamine, 4,4'-methylenebis(cyclohexylamine), and diethyltoluenediamine. The acid-functionalized SWNTs and the amide derivatives were characterized by a set of materials characterization methods including attenuated total reflectance (ATR) FTIR, Raman and solid state (13)C NMR spectroscopy, transmission electron microscopy (TEM), and thermal gravimetry-mass spectrometry (TG-MS).

Oxidative properties and chemical stability of fluoronanotubes in matrixes of binary inorganic compounds.

The chemical stability of fluoronanotubes in selected solid inorganic matrixes has been studied by initially mixing and mechanically grinding the components and subsequently heating them at temperatures ranging from 35 to 600 degrees C. The inorganic compounds selected for matrixes included halides (KBr, KI, Lil, LiBr, LiCl, NaCl, Znl2), oxides (Li2O, Fe2O3, PbO, MnO), lithium peroxide (Li2O2), potassium superoxide (KO2), sulfides (Li2S and ZnS), zinc selenide (ZnSe), lithium nitride (Li3N), and aluminum phosphide (AIP). Solid products, resulting from the proceeding chemical reactions, were analyzed by X-ray diffraction, Raman spectroscopy, and SEM/EDX elemental analysis.

Adsorption of chromium from aqueous solutions by maple sawdust.

This paper presents the data for the effect of adsorbent dose, initial sorbate concentration, contact time, and pH on the adsorption of chromium(VI) on maple sawdust. Batch adsorption studies have been carried out. An empirical relationship has been obtained to predict the percentage chromium(VI) removal at any time for known values of sorbent and initial sorbate concentration.

Covalent sidewall functionalization of single wall carbon nanotubes.

Alkyllithium reagents may be used to attach alkyl groups to the sidewalls of fluoro nanotubes. Thermal gravimetric analysis combined with UV-vis-Nir spectroscopy has been used to provide a quantitative measure of the degree of functionalization. SWNTs prepared using the HiPco process exhibit a higher degree of alkylation than SWNTs from the laser-oven method, indicating that the smaller diameter fluoro tubes are alkylated more readily.

Sidewall functionalization of single-walled carbon nanotubes with organic peroxides.

Single-wall carbon nanotubes (SWNTs) and their fluorinated derivatives (F-SWNTs) were reacted with organic peroxides including benzoyl and lauroyl peroxide to produce phenyl and undecyl sidewall functionalized SWNTs, respectively, which were characterized by Raman, FTIR, and UV-Vis-NIR spectra as well as TGA/MS, TGA/FTIR, and TEM data.

Fluorination of single-wall carbon nanotubes and subsequent derivatization reactions.

This Account focuses on the most recent and systematic efforts in the area of functionalization chemistry of the single-wall carbon nanotubes (SWNTs) which utilizes direct fluorination for the preparation of "fluoronanonotubes" and their subsequent derivatization. The results obtained prove that the addition of fluorine drastically enhances the reactivity of the nanotube side walls. The use of this strategy as a versatile tool for preparation and manipulation of SWNTs with variable side-wall functionalities has been demonstrated.

The role of sawdust in the removal of unwanted materials from water.

Sawdust, a relatively abundant and inexpensive material is currently being investigated as an adsorbent to remove contaminants from water. Chemical substances including dyes, oil, toxic salts and heavy metals can be removed very effectively with the organic material. This article presents a brief review on the role of sawdust in the removal of contaminants.

Ellipsometric method for the measurement of temperature and optical constants of incandescent transition metals.

The development of a unique noncontact temperature measurement device utilizing rotating analyzer ellipsometry is described. The technique circumvents the necessity of spectral emissivity estimation by direct measurement concomitant with radiance brightness. Simultaneous determinations of dielectric constants and refractive indices allow changes in the physical and chemical state of a heated surface to be monitored.

Pyrolysis of organomercury compounds: investigation by the method of matrix isolation.

The method of matrix isolation has been used to investigate mechanisms of gas-phase chemical reactions, in particular the pyrolysis of some organomercury compounds. A molecular beam of pyrolysis products was condensed simultaneously with a large excess of rare gas at temperatures from 5 to 15 degrees K to form a matrix that was subsequently studied by infrared spectroscopy. In the case of C(6)H(5)HgCCl(3), we found that pyrolysis in the temperature range 220-400 degrees C produced mainly dichlorocarbene.