Phenotypical differences of neutrophils patrolling tumour-draining lymph nodes in head and neck cancer.

Background: The complexity and heterogeneity of neutrophils are recognized, especially their roles in modulating inflammation and cancer immune responses. The detailed functions of neutrophils in human tumour-draining lymph nodes (TDLNs), specifically in the context of head and neck cancer, remain inadequately characterized.

Aim: This study aims to delineate the phenotypic diversity of neutrophils in TDLNs, non-tumour-draining lymph nodes (nTDLNs) from patients with oral squamous cell carcinoma (OSCC), and to evaluate their correlation with clinical outcomes.

"High-risk" tumors of the lip treated with external beam radiotherapy and high-dose-rate brachytherapy: Long-term outcome.

Background: Radiotherapy is a well-established treatment for lip cancer, with external radiotherapy (EBRT) or brachytherapy (BT).

Methods: This study evaluated outcome, tumor control, and aesthetics, for 101 patients with carcinoma of the lip, not suitable for surgery, treated with combined EBRT and BT.

Results: Squamous cell carcinoma was seen in 78 patients, basal cell carcinoma in 15, and other histologies in 8 patients.

The transcriptional response of cortical neurons to concussion reveals divergent fates after injury.

Traumatic brain injury (TBI) is a risk factor for neurodegeneration, however little is known about how different neuron types respond to this kind of injury. In this study, we follow neuronal populations over several months after a single mild TBI (mTBI) to assess long ranging consequences of injury at the level of single, transcriptionally defined neuronal classes. We find that the stress responsive Activating Transcription Factor 3 (ATF3) defines a population of cortical neurons after mTBI.

Social contributions to meaning in life: the role of romantic relationship quality, parenting, and gender.

Introduction: The present study tests the association between romantic relationship quality and number of children on meaning in life (i.e., sense of purpose, coherence, and significance) and considers interactions between these constructs and gender.

A day in the life: Couples' everyday communication and subsequent relationship outcomes.

Understanding how communication processes contribute to well-functioning versus distressed couple relationships has relied largely on brief, laboratory-based conversations. Harnessing technological advancements, the present study extends the literature by capturing couples' naturalistic communication over one full day at Time 1 (T1). This study tested associations between data-driven categories of couple communication behaviors and relationship outcomes (i.

Anti-Black violence, discrimination, and sleep difficulties amongst racially diverse Americans: The aftermath of the murder of George Floyd.

Rationale: Following the murders of George Floyd and other Black Americans during the summer of 2020, there was unprecedented exposure to media-disseminated depictions of anti-Black violence. Little is known about the impact of this widespread form of vicarious racism that was pervasive during that historic time.

Objective: The present study applies the concept of vicarious racism to study this secondary exposure to anti-Black violence.

Single-cell analysis of shared signatures and transcriptional diversity during zebrafish development.

During development, animals generate distinct cell populations with specific identities, functions, and morphologies. We mapped transcriptionally distinct populations across 489,686 cells from 62 stages during wild-type zebrafish embryogenesis and early larval development (3-120 h post-fertilization). Using these data, we identified the limited catalog of gene expression programs reused across multiple tissues and their cell-type-specific adaptations.

Evaluating Stacked Methylation Markers for Blood-Based Multicancer Detection.

The ability to detect several types of cancer using a non-invasive, blood-based test holds the potential to revolutionize oncology screening. We mined tumor methylation array data from the Cancer Genome Atlas (TCGA) covering 14 cancer types and identified two novel, broadly-occurring methylation markers at and . To evaluate their performance as a generalized blood-based screening approach, along with our previously reported methylation biomarker, , we rigorously assessed each marker individually or combined.

Loss during COVID-19: Moderating effects of meaning and romantic relationships on anxiety and depressive symptoms.

Objective: The COVID-19 pandemic exacerbated experiences of loss and grief for many individuals and posed a challenging mental health crisis. Compared to studies examining a singular type of loss, the present study investigated the cumulative impacts of COVID-related losses on anxiety and depressive symptoms and examined whether meaning in life, marital status, or relationship quality offered a protective moderating role.

Method: A cross-sectional online survey was conducted approximately 1 year into the pandemic among 434 diverse individuals (32.

A Novel Method Using Fine Needle Aspiration from Tumor-Draining Lymph Nodes Could Enable the Discovery of New Prognostic Markers in Patients with Cutaneous Squamous Cell Carcinoma.

Cutaneous squamous cell cancer (cSCC) is the second most common form of skin cancer, characterized by abnormal, accelerated growth of squamous cells. When caught early, most cSCCs are curable. About 5 percent of the cSCC cases have advanced to such an extent, generally metastatic, that they are far more dangerous, with very poor prognosis and challenging to treat.

The birth of a story: Childbirth experiences, meaning-making, and postpartum adjustment.

The present study investigated how meaning-making around a birth experience predicts relationship quality and parenting stress across the transition to first-time parenthood, a time that many new parents find stressful and challenging. Childbirth experiences may set the stage for these challenges, and how new parents make meaning of childbirth could play a role in their subsequent postpartum adjustment. Meaning-making processes (sense making, benefit finding, and changes in identity) were coded from birth narratives collected from 77 mixed-sex biological parent dyads ( = 154 individuals) shortly after the birth of their first child.

Radiotherapy-Dose Escalated for Large Volume Primary Tumors-And Cetuximab with or without Induction Chemotherapy for HPV Associated Squamous Cell Carcinoma of the Head and Neck-A Randomized Phase II Trial.

Unlabelled: The leading cause of death for patients with HPV associated squamous cell carcinoma of the head and neck (SCCHN) after treatment with chemoradiotherapy (CRT) nowadays is peripheral metastasis. This study investigated whether induction chemotherapy (IC) could improve progression free survival (PFS) and impact on relapse pattern after CRT.

Methods: Eligible patients in this multicenter, randomized, controlled, phase 2 trial had p16-positive locoregionally advanced SCCHN.

Regulatory B cells producing IL-10 are increased in human tumor draining lymph nodes.

The contribution of different immune cell subsets, especially T cells, in anti-tumor immune response is well established. In contrast to T cells, the anti-tumor contribution of B cells has been scarcely investigated. B-cells are often overlooked, even though they are important players in a fully integrated immune response and constitute a substantial fraction of tumor draining lymph nodes (TDLNs) known also as Sentinel Nodes.

Sentinel node-assisted neck dissection in advanced oral squamous cell carcinoma-A new protocol for staging and treatment.

Background: Sentinel lymph node biopsy (SLNB) is used to improve the staging of and guide treatment in patients with early-stage T1-T2 N0 oral squamous cell carcinoma (OSCC). The role of sentinel nodes (SNs) and the use of SN-technique in advanced OSCC (T3-T4 and/or N+) remain to be evaluated. This study investigates the nodal drainage and the rate of positive SNs (SNs+) in all stages of OSCC.

Single-cell analysis of shared signatures and transcriptional diversity during zebrafish development.

During development, animals generate distinct cell populations with specific identities, functions, and morphologies. We mapped transcriptionally distinct populations across 489,686 cells from 62 stages during wild-type zebrafish embryogenesis and early larval development (3-120 hours post-fertilization). Using these data, we identified the limited catalog of gene expression programs reused across multiple tissues and their cell-type-specific adaptations.

Relationship satisfaction, feelings of closeness and annoyance, and linkage in electrodermal activity.

Physiological linkage refers to moment-to-moment, time-linked coordination in physiological responses among people in close relationships. Although people in romantic relationships have been shown to evidence linkage in their physiological responses over time, it is still unclear how patterns of covariation relate to in-the-moment, as well as general levels of, relationship functioning. In the present study with data collected between 2014 and 2017, we capture linkage in electrodermal activity (EDA) in a diverse sample of young-adult couples, generally representative and generalizable to the Los Angeles community from which we sampled.

Tumour-draining lymph nodes in head and neck cancer are characterized by accumulation of CTLA-4 and PD-1 expressing Treg cells.

Introduction: High Tregs infiltration within the tumour microenvironment (TME) of various cancers shows a positive correlation with poor prognosis. Despite the fact that tumour draining lymph nodes (TDLNs) are recognized as key organs playing a crucial role in response to immunotherapy and modulating anti-cancer immunity, the distribution of Tregs and their role in TDLNs remain uncertain thus far. The purpose of this project is to investigate the density of Tregs in TDLNs and non-TDLNs and their expression of immune checkpoint molecules - PD-1 and CTLA-4.

What happens when romantic couples discuss personal loss? Relational, emotional, and physiological impacts.

Does talking about loss with a romantic partner have salutary personal and relationship effects? Prior evidence reveals the benefits of emotional disclosure in couple relationships, yet disclosure about loss has been overlooked in research on couple communication. Using a novel communication paradigm with young-adult heterosexual romantic partners ( = 114 couples), we investigated emotions, physiological arousal (skin conductance responses [SCR]), and relationship closeness when narrating a personal loss and listening to the partner's loss, and compared these loss discussions to discussions about desired relationship changes. Based on partners' self-reports, narrating loss elicited more vulnerable and, unexpectedly, more antagonistic emotions.

Navigation system for percutaneous tracheotomy.

Background: Percutaneous dilatational tracheotomy (PDT) is a well-established method. The prerequisite is to identify anatomical landmarks of the neck. We introduce a three-dimensional navigation system - SafeTrach.

Observed parent-adolescent coalitions and family hostilities during family discussions: Associations with marital aggression.

This study investigated bidirectional associations between observed parent-youth coalitions-wherein one parent and a child align themselves against the other parent-and family hostilities as they evolved in real-time during triadic family conflict discussions. Participants were 102 families with an adolescent child (50% girls, M  = 15.3 years, SD = 0.

Dating Aggression and Observed Behaviors in a Nonconflictual Situation: The Role of Negative Anticipation.

Past observational studies highlight meaningful behavioral differences between aggressive and nonaggressive couples during conflict interactions. However, research is needed on how aggressive couples communicate in other, nonconflictual interactional contexts. This study investigates how dating partners' perpetration of physical aggression relates to observed behaviors during a laboratory-based discussion during which dating couples planned a date together.

Fluctuations in pronoun use in everyday life: Understanding couple aggression in context.

Everyday language use, including the pronouns people choose when speaking to romantic partners, may reflect underlying aspects of relationship functioning and may have important implications for understanding couple conflict and dating aggression more generally. The current study measured couples' hour-to-hour "we," "I," and "you" speech in daily life and examined symmetry in pronoun use, or the extent to which partners mirror each other in the frequency of the pronouns they use. First, we examined associations between symmetry in pronoun use and overall levels of dating aggression.

Romantic partner presence and physiological responses in daily life: Attachment style as a moderator.

The present study investigated whether the presence of a romantic partner in daily life is associated with attenuated sympathetic nervous system responses. Additionally, romantic attachment style was tested as a moderator. For one day, 106 heterosexual young adult dating couples wore ambulatory sensors that monitored electrodermal activity (EDA) - an index of sympathetic arousal.