Grouping of streptomycetes using 16S-ITS RFLP fingerprinting.

A total of 463 Streptomyces and Kitasatospora type strains were screened using 16S-ITS RFLP fingerprinting (combined restriction digest using enzymes BstUI and HaeIII). In total, 59 clusters could be delineated, each comprising multiple strains with nearly identical patterns. Good correlation was found in general with phylogeny, as revealed by 16S rDNA sequencing.

Caenibacterium thermophilum is a later synonym of Schlegelella thermodepolymerans.

Recently, two strains of Schlegelella thermodepolymerans Elbanna et al. 2003 and an independently isolated bacterium, Caenibacterium thermophilum Manaia et al. 2003, were described in parallel as gen.

Thermomonas fusca sp. nov. and Thermomonas brevis sp. nov., two mesophilic species isolated from a denitrification reactor with poly(epsilon-caprolactone) plastic granules as fixed bed, and emended description of the genus Thermomonas.

Previously, 22 aerobic Gram-negative bacteria were isolated from biofilms growing on granules of the synthetic polyester poly(epsilon-caprolactone); the granules were used as a fixed bed in a denitrification reactor. All the strains showed similar fatty acid profiles. The 16S rRNA gene sequences of five strains were phylogenetically related to Thermomonas spp.

Kerstersia gyiorum gen. nov., sp. nov., a novel Alcaligenes faecalis-like organism isolated from human clinical samples, and reclassification of Alcaligenes denitrificans Rüger and Tan 1983 as Achromobacter denitrificans comb. nov.

A polyphasic taxonomic study was performed on nine isolates recovered from various human clinical samples. Phenotypically, these isolates resembled Alcaligenes faecalis. Whole-cell protein analysis distinguished two different species, and this was confirmed by DNA-DNA hybridizations.

Taxonomic study of Cellvibrio strains and description of Cellvibrio ostraviensis sp. nov., Cellvibrio fibrivorans sp. nov. and Cellvibrio gandavensis sp. nov.

Thirty-one cellulolytic bacterial isolates from soils that were phenotypically very similar and phylogenetically highly related to Cellvibrio strains were further characterized using a polyphasic taxonomic approach. By using repetitive extragenic palindromic DNA-PCR fingerprinting, six different fingerprints could be recognized among the isolates. Representative strains and four reference strains of the genus Cellvibrio were used for DNA-DNA hybridization, which yielded eight DNA hybridization groups at a cut-off level of 70% DNA binding.

Diversity of 746 heterotrophic bacteria isolated from microbial mats from ten Antarctic lakes.

Microbial mats, growing in Antarctic lakes constitute unique and very diverse habitats. In these mats microorganisms are confronted with extreme life conditions. We isolated 746 bacterial strains from mats collected from ten lakes in the Dry Valleys (lakes Hoare and Fryxell), the Vestfold Hills (lakes Ace, Druzhby, Grace, Highway, Pendant, Organic and Watts) and the Larsemann Hills (lake Reid), using heterotrophic growth conditions.

Phyllobacterium myrsinacearum (subjective synonym Phyllobacterium rubiacearum) emend.

The taxonomic relationships between Phyllobacterium myrsinacearum LMG 2t2T, Phyllobacterium rubiacearum LMG 1t1T and two Phyllobacterium strains representing a collection of isolates from sugar-beet roots were investigated by means of fatty acid analysis and DNA-DNA hybridization. The strains showed very similar fatty acid compositions and more than 96% DNA reassociation to each other. On the basis of these results and available data from the literature, it is proposed that P.

Fulvimonas soli gen. nov., sp. nov., a gamma-proteobacterium isolated from soil after enrichment on acetylated starch plastic.

Six deep-yellow-pigmented strains were isolated from soil after enrichment on plasticized acetylated starch granules as a source of carbon. They showed very similar and unique fatty acid profiles, consisting almost exclusively of branched fatty acids. The strains consisted of small, motile rods, were oxidase- and catalase-positive, did not ferment sugars and were able to depolymerize starch and suspended acetylated starch in overlayer plates.