Capillary electrophoresis for pharmaceutical analysis.

This chapter describes the application of capillary electrophoresis (CE) to pharmaceutical analysis. The areas of pharmaceutical analysis covered are enantiomer separation, analysis of small molecules such as amino acids or drug counter-ions, pharmaceutical assay, related substances determinations, and physiochemical measurements such as log P and pKa of compounds. The different electrophoretic modes available and their advantages for pharmaceutical analysis are described.

Optimisation and use of water-in-oil MEEKC in pharmaceutical analysis.

Water-in-oil microemulsion electrokinetic chromatography has been applied to the separation of a range of acids, bases and neutrals and is especially suitable for very water-insoluble drug compounds. A number of operating parameters were evaluated. An optimised set of operating conditions allowed separation of a range of pharmaceutical formulations containing water-insoluble compounds.

Recent advances in microemulsion electrokinetic chromatography.

Microemulsion electrokinetic chromatography (MEEKC) is an electrodriven separation technique. Separations are typically achieved using oil-in-water microemulsions, which are composed of nanometre-sized droplets of oil suspended in aqueous buffer. The oil droplets are coated in surfactant molecules and the system is stabilised by the addition of a short-chain alcohol cosurfactant.

The use of novel water-in-oil microemulsions in microemulsion electrokinetic chromatography.

We describe the novel use of water-in-oil (W/O) microemulsions to achieve unique separations in microemulsion electrokinetic chromatography (MEEKC). The choice and concentration of the buffer type, surfactant and co-surfactant were all examined and optimized. Separations of a range of neutral and acidic analytes was shown to be markedly different to that obtained by (oil-in-water) O/W MEEKC.