Mechanism of Intracellular Elemental Sulfur Oxidation in , Where Persulfide Dioxygenase Plays a Key Role.

Representatives of the colorless sulfur bacteria of the genus use reduced sulfur compounds in the processes of lithotrophic growth, which is accompanied by the storage of intracellular sulfur. However, it is still unknown how the transformation of intracellular sulfur occurs in representatives. Annotation of the genome of D-402 did not identify any genes for the oxidation or reduction of elemental sulfur.

Wastewater Treatment with Bacterial Representatives of the Morphotype.

Bacteria of the morphotype, comprising the genera , and , are frequently encountered in domestic and industrial wastewater treatment systems, but they are usually not clearly differentiated due to the marked similarity in their morphologies. Methods ranging from light microscopy, FISH and PCR to modern high-throughput sequencing are used to identify them. The development of these bacteria in wastewater treatment systems has both advantages and disadvantages.

Phylogeny and Metabolic Potential of New Giant Sulfur Bacteria of the Family from Coastal-Marine Sulfur Mats of the White Sea.

The family is currently represented by 25 genera in the Genome Taxonomy Database, of which only 6 have a definite taxonomic status. Two metagenome-assembled genomes (MAGs), WS_Bin1 and WS_Bin3, were assembled from metagenomes of the sulfur mats coating laminaria remnants in the White Sea. Using the obtained MAGs, we first applied phylogenetic analysis based on whole-genome sequences to address the systematics of , which clarify the taxonomy of this family.

and : New Molecular Markers for Phylogenetic and Biodiversity Studies of the Genus .

Currently, the phylogeny of the genus is based on comparative whole genome analysis because of the high homology of the 16S ribosomal RNA gene sequences within the genus. We analyzed the possibility of using various conservative genes as phylogenetic markers for the genus . We found that the levels of similarity of the nucleotide sequences of the tRNA(Ile)-lysidine synthase () and the β subunit of RNA polymerase () genes are in good agreement with the average nucleotide identity (ANI) values between the genomes of various representatives of the genus .

Metagenomics Revealed a New Genus ' Thiocaldithrix dubininis' gen. nov., sp. nov. and a New Species ' Thiothrix putei' sp. nov. in the Family , Some Members of Which Have Traits of Both Na- and H-Motive Energetics.

Two metagenome-assembled genomes (MAGs), GKL-01 and GKL-02, related to the family have been assembled from the metagenome of bacterial mat obtained from a sulfide-rich thermal spring in the North Caucasus. Based on average amino acid identity (AAI) values and genome-based phylogeny, MAG GKL-01 represented a new genus within the family. The GC content of the GKL-01 DNA (44%) differed significantly from that of other known members of the genus (50.

History of the Study of the Genus : From the First Enrichment Cultures to Pangenomic Analysis.

Representatives of the genus are filamentous, sulfur-oxidizing bacteria found in flowing waters with counter-oriented sulfide and oxygen gradients. They were first described at the end of the 19th century, but the first pure cultures of this species only became available 100 years later. An increase in the number of described species at the beginning of the 21st century shows that the classical phylogenetic marker, 16S rRNA gene, is not informative for species differentiation, which is possible based on genome analysis.

Correction: Ravin et al. Two New Species of Filamentous Sulfur Bacteria of the Genus , sp. nov. and ' Thiothrix sulfatifontis' sp. nov. 2022, , 1300.

The authors wish to make the following corrections to this paper [...

Two New Species of Filamentous Sulfur Bacteria of the Genus , sp. nov. and ' Thiothrix sulfatifontis' sp. nov.

The metagenome of foulings from sulfidic spring "Serovodorodny" (Tatarstan, Russia), where members of the genus was observed, was sequenced. Representatives of the phyla , and dominated in the microbial community. The complete genome of sp.

Comparative Genome Analysis of the Genus Involving Three Novel Species, sp. nov. Ku-5, sp. nov. AS and " Thiothrix anitrata" sp. nov. A52, Revealed the Conservation of the Pathways of Dissimilatory Sulfur Metabolism and Variations in the Genetic Inventory for Nitrogen Metabolism and Autotrophic Carbon Fixation.

Two strains of filamentous, colorless sulfur bacteria were isolated from bacterial fouling in the outflow of hydrogen sulfide-containing waters from a coal mine ( sp. Ku-5) and on the seashore of the White Sea ( sp. AS).

Reclassification of Sphaerotilus natans subsp. sulfidivorans Gridneva et al. 2011 as Sphaerotilus sulfidivorans sp. nov. and comparative genome analysis of the genus Sphaerotilus.

Filamentous iron oxides accumulating bacteria Sphaerotilus natans subsp. natans and S. natans subsp.

Genomic and Metabolic Insights into Two Novel Species from Enhanced Biological Phosphorus Removal Systems.

Two metagenome-assembled genomes (MAGs), obtained from laboratory-scale enhanced biological phosphorus removal bioreactors, were analyzed. The values of 16S rRNA gene sequence identity, average nucleotide identity, and average amino acid identity indicated that these genomes, designated as RT and SSD2, represented two novel species within the genus , ' Thiothrix moscowensis' and ' Thiothrix singaporensis'. A complete set of genes for the tricarboxylic acid cycle and electron transport chain indicates a respiratory type of metabolism.

The role of the "Thiodendron" consortium in postulating the karyomastigont chimaera of the endosymbiosis theory by Lynn Margulis.

The endosymbiosis theory of the origin of eukaryotic cell was first proposed more than a hundred years ago. In the second half of the 20th century, Lynn Margulis suggested a new interpretation of the origin of the nucleus in modern eukaryotes. The background was the study of the consortium "Thiodendron", a symbiotic bacterial community, which includes anaerobic aerotolerant motile spirochaetes and sulfidogenic bacteria (sulfidogens) of vibrioid form with a fermentation type of metabolism.

Description of sp. nov. and reclassification of as gen. nov., comb. nov.

A novel obligately anaerobic spirochete strain K2 was isolated from bottom marine sediments at Crater Bay of Yankicha Island (Kuril Islands, Russia). Strain K2 had helical shape and Gram-negatively stained. The optimal growth conditions were as follows: the optimum temperature was 28-30 °C with range 5-34 °C; optimal pH at 7.

Comparative Genomics of Strains D-401 and D-402 with Contrasting Physiology but Extremely High Level of Genomic Identity.

Representatives of filamentous colorless sulfur-oxidizing bacteria often dominate in sulfide biotopes, preventing the diffusion of toxic sulfide into the water column. One of the most intriguing groups is a recently described including strains D-401 and D-402. Both strains have identical genes encoding enzymes which are involved in the oxidation of hydrogen sulfide and thiosulfate.

Complete Genome Sequences and Methylome Analysis of Two Environmental .

Here, we report the finished closed genomes of two environmental bacteria, K2 and P (formally known as P). In addition, we provide methylation data and the associated enzymes predicted and confirmed to be responsible for each modified motif.

Complete Genome Sequence and Methylome Analysis of Sphaerotilus natans subsp. D-507.

subsp. D-507 is an environmental isolate from a sulfate spring in the northern Caucasus region of Russia. This heterotrophic bacterium is involved in the oxidation of reduced sulfur derivatives.

Genomics and Biochemistry of Metabolic Pathways for the C Compounds Utilization in Colorless Sulfur Bacterium D-402.

The metabolic pathways of one-carbon compounds utilized by colorless sulfur bacterium D-402 were revealed based on comprehensive analysis of its genomic organization, together with physiological, biochemical and molecular biological approaches. Strain D-402 was capable of aerobic methylotrophic growth with methanol as a sole source of carbon and energy and was not capable of methanotrophic growth because of the absence of genes of methane monooxygenases. It was established that methanol can be oxidized to CO in three consecutive stages.

Complete Genome Sequence of the Freshwater Bacterium Beggiatoa leptomitoformis Strain D-401.

Here, we report the complete closed genome sequence and methylome analysis of strain D-401 (DSM 14945, UNIQEMU 779), which is quite different from the previously described neotype strain D-402 (DSM 14946, UNIQEM U 779) with regard to morphology and lithotrophic growth in the presence of thiosulfate.

Whole-Genome Sequence and Methylome Analysis of the Freshwater Colorless Sulfur Bacterium D3.

In this report, we announce the availability of a whole-genome sequence and methylome analysis of strain D3.

Beggiatoa leptomitoformis sp. nov., the first freshwater member of the genus capable of chemolithoautotrophic growth.

A strain of filamentous sulfur bacteria was isolated from freshwater spring contaminated with residential and agricultural wastewater in Moscow region, Russia. According to the results of phylogenetic analysis, strain D-402T belonged to the genus Beggiatoa within the family Beggiatoaceae of the class Gammaproteobacteria. Within the genus Beggiatoa, strain D-402T was most closely related to Beggiatoa alba strains.

Genomic insights into metabolic versatility of a lithotrophic sulfur-oxidizing diazotrophic Alphaproteobacterium Azospirillum thiophilum.

Diazotrophic Alphaproteobacteria of the genus Azospirillum are usually organotrophs, although some strains of Azospirillum lipoferum are capable of hydrogen-dependent autotrophic growth. Azospirillum thiophilum strain was isolated from a mineral sulfide spring, a biotope highly unusual for azospirilla. Here, the metabolic pathways utilized by A.

Complete Genome Sequence of a Strain of Azospirillum thiophilum Isolated from a Sulfide Spring.

We report the complete, closed genome sequence and complete methylome of Azospirillum thiophilum strain BV-S(T).

Complete Genome Sequence of the Freshwater Colorless Sulfur Bacterium Beggiatoa leptomitoformis [corrected] Neotype Strain D-402T.

In this report, we announce the availability of a complete closed genome sequence and methylome analysis of Beggiatoa leptomitiformis neotype strain D-402(T) (DSM 14946, UNIQEM U 779).

Spirochaeta sinaica sp. nov., a halophilic spirochaete isolated from a cyanobacterial mat.

A strain of free-living obligately anaerobic, halophilic spirochaete, SLT, was isolated from a sample of a cyanobacterial mat of the hypersaline Solar Lake, Sinai shore. The strain had motile helical cells, 0.35-0.