Publications by authors named "Margarita Belinite"
The 5' cap and 3' poly(A) tail of mRNA are known to synergistically stimulate translation initiation via the formation of the cap•eIF4E•eIF4G•PABP•poly(A) complex. Most mRNA sequences have an intrinsic propensity to fold into extensive intramolecular secondary structures that result in short end-to-end distances. The inherent compactness of mRNAs might stabilize the cap•eIF4E•eIF4G•PABP•poly(A) complex and enhance cap-poly(A) translational synergy.
View Article and Find Full Text PDFThe ribosome is a complex cellular machinery whose solved structure allowed for an incredible leap in structural biology research. Different ions bind to the ribosome, stabilizing inter-subunit interfaces and structurally linking rRNAs, proteins, and ligands. Besides cations such as K and Mg , polyamines are known to stabilize the folding of RNA and overall structure.
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- Cryo-electron microscopy (cryo-EM) is a key technique in structural biology for examining protein synthesis via ribosomes, requiring stable and pure samples for high-resolution images.
- A new protocol has been developed for purifying stable 30 S ribosomal subunits from a Gram-positive bacterium, highlighting the importance of spermidine in maintaining the structure.
- The study reveals that ribosomes in the presence of spermidine achieve resolutions of 3.4 to 3.6 Å, while those without it can only reach 5.3 Å, underscoring spermidine's role in stabilizing critical ribosomal structures.