Larvicidal and Adulticidal Activity of Essential Oils from Four Cuban Plants against Three Mosquito Vector Species.

Mosquitoes are one of the main vectors of many important diseases and their degree of resistance to chemical insecticides has increased. Nowadays, it has become crucial to identify novel plant larvicides with an eco-friendly impact. The components of essential oils from Jacq.

Development and Intrapuparial Characterization of Hydrotaea aenescens (Diptera: Muscidae) Raised at Different Temperatures Under Laboratory Conditions.

Species belonging to the Muscidae family are important in the medical-sanitary and forensic fields due to their biology. The intrapuparial stage of Hydrotaea aenescens (Wiedemann, 1830), which represents about 50% of the immature development cycle in muscoid Diptera, has not yet been studied in detail. This study identifies and describes the main temporal morphological changes that occur in the pupal body of H.

Ultrastructural and pathogenicity of Brevibacillus laterosporus against sinantropic muscoid dipterans.

Brevibacillus laterosporus has entomopathogenic potential against several orders of insects and its wide bioactivity is associated with a variety of strain-specific molecules. In order to avoid the use of synthetic insecticides, along with the need to control insect pests, microbial control has been widely used. Muscoid dipterans are known for their medical-veterinary and sanitary importance, and synanthropy.

Ultrastructure of the Immature Stages of Musca domestica (Diptera: Muscidae: Muscinae).

Musca domestica (Linnaeus, 1758) is a muscoid species that is widespread throughout the world and acts as a mechanical vector of different enteropathogens primarily in underdeveloped countries. The adult and its immature forms are associated with decaying organic matter and can be seen visiting human corpses and animal carcass, and the larvae can also cause an infestation on human and animal wounds, feeding on the tissues. These characteristics make them have a forensic significance and, mainly, a great sanitary importance.

Larvicidal and adulticidal effects and ultrastructural changes of larvae midgut epithelium of Musca domestica (Diptera: Muscidae) fed with Bacillus thuringiensis var. kyushuensis.

Introduction: Musca domestica is resistant to many insecticides; hence, biological control is a suitable alternative.

Methods: We evaluated the lethality of strain Btk176 towards the larval and adult M. domestica and the histopathological effects in the larvae midgut.

Laboratory evaluation of Brevibacillus laterosporus strains as biocidal agents against Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) larvae.

The biocidal activity of three strains of Brevibacillus laterosporus upon the post-embryonic developmental stages of Chrysomya megacephala was evaluated. Bioassays were performed to verify lethal and sub-lethal effects including ultra-structural changes in the midgut. Among the strains assayed, Shi3 presented the highest larval mortality rates, achieving 70% at a concentration of 1×10 spores/g of diet.

Ultrastructure analysis of the immature stages of Ravinia belforti (Diptera: Sarcophagidae), a species of medical-veterinary and forensic importance, by scanning electron microscopy.

The postmortem interval is related to the age of immature species of flies found on corpses and can be estimated using data available in the literature on the biology of the species. The flesh fly Ravinia belforti is a carrier of enteric pathogens that can affect human and animal health as well as being of forensic importance. As the morphology of many immature Sarcophagidae is unknown, these immature forms must be collected and characterized after the emergence of the adult male.

Ultrastructure of immature stages of the black dump fly: Ophyra aenescens (Wiedemann, 1830) (Diptera: Muscidae: Azeliinae).

Ophyra aenescens (black dump fly) originally belonged to the New World, however, now it is spread worldwide. This fly is a mechanical vector of some pathogenic microorganisms and eggs of the human botfly (Dermatobia hominis). The adults are associated with decaying matter and the immature stages colonize animal and human corpses.

Effect of age on cuticular hydrocarbon profiles in adult Chrysomya putoria (Diptera: Calliphoridae).

A species-specific complex mixture of highly stable cuticular hydrocarbons (CHCs) covers the external surface of all insects. Components can be readily analyzed by gas chromatography coupled to mass spectrometry (GC-MS) to obtain a cuticular hydrocarbon profile, which may be used as an additional tool for the taxonomic differentiation of insect species and also for the determination of the age and sex of adult and immature forms. We used GC-MS to identify and quantify the CHCs of female and male Chrysomya putoria (Wiedemann, 1818) (Diptera: Calliphoridae) from one to five days old.

Preliminary screening of the larvicidal effect of Brevibacillus laterosporus strains against the blowfly Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae).

Introduction: This study evaluated whether different strains of Brevibacillus laterosporus could be used to control larvae of the blowfly Chrysomya megacephala, a pest that affects both human and animal health.

Methods: Mortality rates were recorded after 1-mL suspensions of sporulated cells of 14 different strains of B. laterosporus were added to 2.

Chemical composition and insecticidal activity of Cymbopogon citratus essential oil from Cuba and Brazil against housefly.

Essential oil of Cymbopogon citratus collected from Brazil and Cuba was tested to a chemical characterization and then was tested on the post-embryonic development of Musca domestica. The chemical composition analysis by GC-MS of the oils from Brazil/Cuba allowed the identification of 13 and 12 major constituents respectively; nine of them common to both. In the both oils, the main components were the isomers geranial and neral, which together form the compound citral.

Detection and preliminary physico-chemical properties of antimicrobial components in the native excretions/secretions of three species of Chrysomya (Diptera, Calliphoridae) in Brazil.

Antibiotic-resistant bacteria in hospitals and communities increasingly threaten public health in Brazil and the rest of the World. There is an urgent need for additional antimicrobial drugs. Calliphorid blowfly larvae are a rich source of antimicrobial factors but the potential of Neotropical species has been neglected.

Ultrastructural studies of some character of Diptera (Muscidae) of forensically importance.

Insects are important in the decomposition of cadavers. In the field of forensic entomology, the taxonomic identification is essential to proceed to any procedure. The use of these insects in medico criminal investigation is the object of forensic entomological studies; the flies are generally attracted to cadavers and one of the most important contributions is to estimate the postmortem interval.

Ultrastructure of immature stages of Cochliomyia macellaria (Diptera: Calliphoridae), a fly of medical and veterinary importance.

Cochliomyia macellaria (Diptera: Calliphoridae) is known as the secondary screwworm because it causes secondary or facultative myiasis when the larvae feed on necrotic tissues. This fly has a significant medical and veterinary importance since it has been reported to transport eggs of Dermatobia hominis (human botfly), which can cause significant economic losses to livestock. Since this screwworm has been collected colonizing both pig carcasses and human cadavers, it is considered one of the most important species for forensic entomology studies.

Ultrastructure of immature stages of Lucilia cuprina (Diptera: Calliphoridae) using scanning electron microscopy.

The blowfly Lucilia cuprina is distributed worldwide and is a mechanical vector of pathogens. It can cause myiasis in humans and is strongly related to forensic entomology, as it is frequently found on human and animal corpses. However, most of the L.

Cuticular hydrocarbons as a tool for the identification of insect species: puparial cases from Sarcophagidae.

The external surface of all insects is covered by a species-specific complex mixture of highly stable, very long chain cuticular hydrocarbons (CHCs). Gas chromatography coupled to mass spectrometry was used to identify CHCs from four species of Sarcophagidae, Peckia (Peckia) chrysostoma, Peckia (Pattonella) intermutans, Sarcophaga (Liopygia) ruficornis and Sarcodexia lambens. The identified CHCs were mostly a mixture of n-alkanes, monomethylalkanes and dimethylalkanes with linear chain lengths varying from 23 to 33 carbons.

Ultrastructure of immature stages of Peckia (Euboetcheria) collusor (Diptera: Sarcophagidae).

Peckia (Euboettcheria) collusor (Diptera: Sarcophagidae) is a necrophagous insect and represents an important role in forensic entomology, as it is always collected at animal/human corpses. Immature sarcophagids are extremely similar in morphological aspects and can be found colonizing both fresh and mummified bodies. Flesh fly larvae are reported to cause accidental myiasis.

How photoperiods affect the immature development of forensically important blowfly species Chrysomya albiceps (Calliphoridae).

The purpose of this study was to verify the influence of different photoperiods on larval body weight, post-embryonic development, and viability of Chrysomya albiceps. The bioassays were performed in acclimatized chamber at 27 ± 1 °C and 60 ± 10 % relative humidity regulated by three different light and dark cycles for a period of 24 h-12:12, 24:00, and 00:24. Four replications with 50 newly hatched larvae each were used per photoperiod.

Ultrastructure of larvae and puparia of the blowfly Chrysomya megacephala (Diptera: Calliphoridae).

Chrysomya megacephala is a forensic important fly, and its immature forms also cause myiasis. The adults are the first insects to reach a carcass and can oviposit just a few hours after arrival. Therefore, the knowledge of immature stages of flies is essential for correct identification of the species found on corpses.

Ultrastructure of immature stages of the blowfly Chrysomya putoria (Wiedemann, 1818) (Diptera: Calliphoridae).

Forensic entomology is an area of science that serves as a tool in crime scene investigations. Usually, flies are the first insects to reach a carcass and can oviposit just a few hours after arrival. Therefore, the knowledge of immature stages is essential for correct identification of the species found on corpses.

Bioactivity of latex from Euphorbia splendens var. hislopii (Euphorbiaceae) on post-embryonic development of Megaselia scalaris (Phoridae).

Larvae of Megaselia scalaris (Loew, 1866) feed on a wide range of decomposing organic matter and present a great importance to public health. This study evaluated the effect of crude latex extract from Euphorbia splendens var. hislopii (Euphorbiaceae) on post-embryonic development time of M.