Publications by authors named "Margareta Kroner"
Automated high content screening for phosphoinositide 3 kinase inhibition using an AKT 1 redistribution assay.
Comb Chem High Throughput Screen
June 2006
Article Synopsis
- High Content Screening (HCS) is an advanced method that combines fluorescence imaging with automated analysis to examine how test compounds affect cells in disease models, specifically focusing on type I PI3K activity in CHO cells.
- The research utilizes Chinese Hamster Ovary (CHO) cells genetically modified to express human insulin receptors and a fluorescent protein, allowing for the observation of changes in the cell's response to insulin-like growth factor-1 stimulation.
- The study introduces a robust assay for measuring PI3K activity with minimal variability, where the response to PI3K inhibitors can be accurately tested, showing that PI3Kalpha is predominantly activated in this cellular context.
Time-resolved fluorescence measurements using microlens array and area imaging devices.
Comb Chem High Throughput Screen
February 2004
Time-resolved fluorescence (TRF) assay formats are frequently used technologies in high-throughput screening. In this article, we have characterised the novel Plate::Vision(2) 96-microlens array reader (Carl Zeiss Jena GmbH, Germany) and compared it to the novel LEADseeker Generation IV multimodality imaging system (LEADseeker Gen IV; Amersham Biosciences UK Ltd., UK) for applications in the TRF mode.
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