Evaluation of a fibrillin 2 gene haplotype associated with hip dysplasia and incipient osteoarthritis in dogs.

Objective: To determine whether a mutation in the fibrillin 2 gene (FBN2) is associated with canine hip dysplasia (CHD) and osteoarthritis in dogs.

Animals: 1,551 dogs. Procedures-Hip conformation was measured radiographically.

Evaluation of quantitative trait loci for hip dysplasia in Labrador Retrievers.

Objective: To identify the quantitative trait loci (QTL) that contribute to hip dysplasia in dogs.

Animals: 192 Labrador Retrievers.

Procedures: Hip dysplasia was measured by use of the Norberg angle (NA), dorsolateral subluxation (DLS) score, and distraction index (DI).

The long (and winding) road to gene discovery for canine hip dysplasia.

Hip dysplasia is a common inherited trait of dogs that results in secondary osteoarthritis. In this article the methods used to uncover the mutations contributing to this condition are reviewed, beginning with hip phenotyping. Coarse, genome-wide, microsatellite-based screens of pedigrees of greyhounds and dysplastic Labrador retrievers were used to identify linked quantitative trait loci (QTL).

In vitro analysis of nonthermal plasma as a disinfecting agent.

Objective: To determine the effect of nonthermal plasma on Staphylococcus aureus, fibroblasts in monolayer culture, and clean and contaminated skin explants.

Sample Population: Normal skin from euthanized horses.

Procedures: S aureus organisms were plated and treated with nonthermal plasma followed by bacterial culture to assess viability.

Quantitative trait loci for hip dysplasia in a cross-breed canine pedigree.

Canine hip dysplasia is a common developmental inherited trait characterized by hip laxity, subluxation or incongruity of the femoral head and acetabulum in affected hips. The inheritance pattern is complex and the mutations contributing to trait expression are unknown. In the study reported here, 240 microsatellite markers distributed in 38 autosomes and the X chromosome were genotyped on 152 dogs from three generations of a crossbred pedigree based on trait-free Greyhound and dysplastic Labrador Retriever founders.

The potential and limitations of cartilage-specific (V+C)(-) fibronectin and cartilage oligomeric matrix protein as osteoarthritis biomarkers in canine synovial fluid.

Objective: To determine if levels of the cartilage-specific (V+C)(-) fibronectin isoform in the synovial fluid is associated with cartilage change during osteoarthritis.

Design: Synovial fluid was collected from 26 healthy dogs presenting to the Orthopedic Surgery Clinic with unilateral cranial cruciate rupture, 22 control dogs, and 13 dogs from a colony maintained for the study of canine hip dysplasia. Total fibronectin, (V+C)(-) fibronectin, and cartilage oligomeric matrix protein (COMP) were quantitated by ELISA assays.

Evaluation of multiple radiographic predictors of cartilage lesions in the hip joints of eight-month-old dogs.

Objective: To determine the radiographic methods that best predict the development of osteoarthritis in the hip joints of a cohort of dogs with hip dysplasia and unaffected dogs.

Animals: 205 Labrador Retrievers, Greyhounds, and Labrador Retriever-Greyhound crossbred dogs.

Procedure: Pelvic radiography was performed when the dogs were 8 months old.