The putative type 4 secretion system effector BspD is involved in maintaining envelope integrity of the pathogen .

Unlabelled: Brucellosis is a debilitating disease caused by the Gram-negative, facultative intracellular zoonotic pathogen En route to its intracellular replicative niche, encounters various stressful environments that may compromise envelope integrity. Here we show that the proposed type 4 secretion system (T4SS) effector BspD is a conserved protein of the Rhizobiales, which does not show signs of co-evolution with the presence of a T4SS or a certain lifestyle. We further present data indicating that BspD is critical for the envelope integrity of in the stationary phase and in the presence of EDTA, a compound known to destabilize the outer membrane.

Antibiotic persistence of intracellular Brucella abortus.

Background: Human brucellosis caused by the facultative intracellular pathogen Brucella spp. is an endemic bacterial zoonosis manifesting as acute or chronic infections with high morbidity. Treatment typically involves a combination therapy of two antibiotics for several weeks to months, but despite this harsh treatment relapses occur at a rate of 5-15%.

A Role for the VPS Retromer in Intracellular Replication Revealed by Genomewide siRNA Screening.

, the agent causing brucellosis, is a major zoonotic pathogen with worldwide distribution. resides and replicates inside infected host cells in membrane-bound compartments called containing vacuoles (BCVs). Following uptake, resides in endosomal BCVs (eBCVs) that gradually mature from early to late endosomal features.

Virulence-associated protein A from Rhodococcus equi is an intercompartmental pH-neutralising virulence factor.

Professional phagocytic cells such as macrophages are a central part of innate immune defence. They ingest microorganisms into membrane-bound compartments (phagosomes), which acidify and eventually fuse with lysosomes, exposing their contents to a microbicidal environment. Gram-positive Rhodococcus equi can cause pneumonia in young foals and in immunocompromised humans.