Alterations of erythrocyte structure and cellular susceptibility in patients with chronic renal failure: effect of haemodialysis and oxidative stress.

The aim of this study was to investigate erythrocytes rheological behaviour, membrane dynamics and erythrocytes susceptibility to disintegration upon strong oxidative stress induced by dialysis or by external H(2)O(2) among patients with CRF. EPR spectrometry was used to investigate alterations in physical state of cellular components. Generated ROS production induced: (1) significant increase of membrane fluidity in CRF erythrocytes treated with H(2)O(2) (p<0.

[Blood serum and neutrophil amino acid concentrations during hemodialysis].

The abnormal amino acid profile and altered distribution of some amino acids between the extra- and intracellular compartments is currently recognized as a part of metabolism abnormalities in hemodialyzed uremic patients associated with prognosis. The aim of this study was to investigate the pattern of HD influence on the extra- and intracellular amino acid concentrations in neutrophil homogenates and in blood serum. Before HD, the concentrations of arginine (64 vs 33 micromol/l) and cysteine (70 vs 40 micromol/l) were significantly higher in the uremic blood serum, but the concentrations of five amino acids, i.

[Physiopathology of advanced age].

The paper presents basic changes in the cell structure and function and organ dysfunctions related to advanced age.

Increased hydrogen peroxide in the exhaled breath of uraemic patients unaffected by haemodialysis.

Background: Uraemia is accompanied by conditions favouring the rise of H2O2 activity in body fluids. This results from the increased release of H2O2 by polymorphonuclear leukocytes and decreased plasma glutathione peroxidase activity. The purpose of this study was to determine if patients on chronic haemodialysis (HD) exhale more H2O2 than healthy individuals, and if dialysis affects breath H2O2 content.

Effects of uraemia and haemodialysis on neutrophil apoptosis and expression of apoptosis-related proteins.

Background: In haemodialysis (HD) patients, it is unclear whether increased apoptosis of neutrophils is due to uraemia or HD itself. The purpose of the current study was to assess the effect of uraemia and HD on the rate of apoptosis and apoptosis-related protein expression in whole blood neutrophils.

Methods: We employed a whole-blood micromethod to test spontaneous apoptosis and expression of apoptosis-regulating proteins in cultured neutrophils from uraemic patients (pre-HD), HD patients and healthy controls.

Blood serum and neutrophil L-arginine concentrations and nitric oxide release by neutrophils in chronic uremic patients and healthy persons.

Background: Plasma levels of L-arginine have previously been evaluated in several studies to reveal either down- or up-regulation of the L-arginine/NO pathway in chronic uremia.

Material/methods: We studied L-arginine plasma levels along with intracellular neutrophil concentration and NO release by peripheral blood neutrophils in patients with chronic uremia.

Results: The L-arginine plasma concentration was found to be significantly higher (64.

Left atrial function in patients with renal transplantation.

Background: Chronic renal failure leads to structural changes and cardiac functional abnormalities known as uremic cardiomyopathy. Eliminating excess water and improving the composition of the inner environment leads to at least partial cardiac function improvement, which is reflected primarily in favorable changes in left ventricular indices. The aim of our study was to evaluate left atrial function in patients after renal transplantation and compare them with clinically healthy subjects.