Silencing the Oryza sativa plasma membrane H-ATPase isoform OsA2 affects grain yield and shoot growth and decreases nitrogen concentration.

The plasma membrane (PM) H-ATPase (EC 3.6.1.

Dark septate endophytic fungi increase the activity of proton pumps, efficiency of N recovery from ammonium sulphate, N content, and micronutrient levels in rice plants.

Plants colonised by dark septate endophytic (DSE) fungi show increased uptake of nutrients available in the environment. The objective of the present study was to evaluate the impact of DSE fungi on the activity of proton pumps, nitrogen (N) recovery from ammonium sulphate, and nutrient accumulation in rice plants. Treatments consisted of non-inoculated plants and plants inoculated with two isolates of DSE fungi, A101 and A103.

Isoforms of plasma membrane H(+)-ATPase in rice root and shoot are differentially induced by starvation and resupply of NO₃⁻ or NH₄+.

The goal of this study was to evaluate the effects of nitrogen starvation and resupply in 10 PM H+-ATPase isoforms and the expression of NO₃⁻ and NH₄+ transporters in rice. The net uptake of both forms of NO₃⁻-N or NH₄+-N was increased with its resupply. Resupply of NO₃⁻ resulted in induction of the following PM H+-ATPase isoforms, OsA1, OsA2, OsA5 and OsA7 in the shoots and OsA2, OsA5, OsA7 and OsA8 in the roots.